Lang, Wenjie Yuan, Chaonan Zhu, Biwei Pan, Sijun Liu, Jian Luo, Jie Nie, Shikun Zhu, Qing Lee, Jun-Seok Ge, Jingyan 2024-01-19T20:31:47Z 2024-01-19T20:31:47Z 2021-09-02 2019-03-21 1477-0520 https://pubs.kist.re.kr/handle/201004/120199 "Minimalist" small molecule tagging (MSMT) is a promising approach that easily converts bioactive compounds into affinity-based probes (AfBPs) for proteomic studies. In this work, seven bioactive compounds targeting diversified protein classes were installed with "minimalist" linkers through common reactions to generate the corresponding AfBPs. These probes were evaluated for cell-based protein profiling and target validation. Among them, the entinostat-derived probe EN and the camptothecin-derived probe CA were further utilized in cellular imaging and SILAC-based large-scale target identification. Our extensive studies suggest that the "minimalist" small molecule tagging approach could be expanded to different classes of bioactive compounds for modification into AfBPs as a dual functional tool for both proteomics and cellular imaging. English ROYAL SOC CHEMISTRY PHOTO-CROSS-LINKERS TARGET IDENTIFICATION DISCOVERY CELL PROBES DESIGN KINASE ANTICANCER CHALLENGES INHIBITORS Expanding the "minimalist" small molecule tagging approach to different bioactive compounds Article 10.1039/c8ob03175d 1 ORGANIC & BIOMOLECULAR CHEMISTRY, v.17, no.11, pp.3010 - 3017 ORGANIC & BIOMOLECULAR CHEMISTRY 17 11 3010 3017 scie scopus 000461223700019 2-s2.0-85062816934 Chemistry, Organic Chemistry Article PHOTO-CROSS-LINKERS TARGET IDENTIFICATION DISCOVERY CELL PROBES DESIGN KINASE ANTICANCER CHALLENGES INHIBITORS