Lee, Jieon Kim, Young-kwan Lee, Sangwoo Yoon, Seokjoo Kim, Woo-keun 2024-01-19T20:32:28Z 2024-01-19T20:32:28Z 2021-09-02 2019-03-01 0925-4005 https://pubs.kist.re.kr/handle/201004/120238 MicroRNAs (miRNAs), short non-coding RNAs, have emerged as promising next-generation biomarkers of diverse diseases. In general, conventional quantitative real-time PCR and miRNA microarray methods have been utilized for the quantitative detection of miRNA. However, owing to their complicated procedures and expensive reagents/instruments, these methods cannot be widely applied by medical experts such as doctors and nurses, and are thus limited for practical clinical application. Here, we established a new graphene oxide (GO)-based NET strategy for facile miRNA detection with enhanced sensitivity, which is applicable for point-of-care testing (POCT). The proposed miRNA sensing strategy is fundamentally based on the peroxidase-mimicking DNAzyme (Dz) mediated colorimetric assay in response to target miRNA using rationally designed duplex molecular beacon (dMB). The application of GO as a net in this basic system (designated GONET) allows for gathering of the target-dependently activated Dz, which enhances the sensitivity and extends the applicability for POCT. The GONET system provides clear visualization of the target at the 10(-9) M scale with the naked eye without any complicated amplification steps. English ELSEVIER SCIENCE SA HYBRIDIZATION CHAIN-REACTION PEPTIDE NUCLEIC-ACID CIRCULATING MICRORNAS POTENTIAL BIOMARKER GOLD NANOPARTICLES DNA PLATFORM CANCER STRAND ASSAY Graphene oxide-based NET strategy for enhanced colorimetric sensing of miRNA Article 10.1016/j.snb.2018.11.149 1 SENSORS AND ACTUATORS B-CHEMICAL, v.282, pp.861 - 867 SENSORS AND ACTUATORS B-CHEMICAL 282 861 867 scie scopus 000455090000103 2-s2.0-85057491803 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article HYBRIDIZATION CHAIN-REACTION PEPTIDE NUCLEIC-ACID CIRCULATING MICRORNAS POTENTIAL BIOMARKER GOLD NANOPARTICLES DNA PLATFORM CANCER STRAND ASSAY miRNA detection Graphene oxide Colorimetric method Paper sensor Point of care testing