Shin, Ji-Sun Han, Hee-Soo Lee, Seung-Bin Myung, Da-bin Lee, Keunsuk Lee, Sun Hee Kim, Hyoung Ja Lee, Kyung-Tae 2024-01-19T20:33:24Z 2024-01-19T20:33:24Z 2021-09-02 2019-03 0918-6158 https://pubs.kist.re.kr/handle/201004/120289 Hydrangea serrata (THUMB.) SER. (Hydrangeaceae) leaves have been used as herbal teas in Korea and Japan. The objective of this study was to identify anti-photoaging compounds in aqueous EtOH extract prepared from leaves of H. serrata and their effects on UVB-irradiated Hs68 human foreskin fibroblasts. Phytochemical study on H. serrata leaves led to the isolation and characterization of ten compounds: hydrangenol, thunberginol A, thunberginol C, hydrangenoside A, hydrangenoside C, cudrabibenzyl A, 2,3,4'-trihydroxystilbene, thunberginol F, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-galactopyranoside, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-glucopyranoside. Cudrabibenzyl A, 2,3,4'-trihydroxystilbene, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-galactopyranoside, quercetin 3-O-beta-D-xylopyranosyl (1-2)-beta-D-glucopyranoside were firstly isolated from H. serrata. We estimated the effects of 10 compounds on cell viability and production of pro-collagen Type I, matrix metalloproteinase (MMP)-1, and hyaluronic acid (HA) after UVB irradiation. Of these compounds, hydrangenol showed potent preventive activities against reduced cell viability and degradation of pro-collagen Type I in UVB-irradiated Hs68 fibroblasts. Hydrangenol had outstanding inductive activities on HA production. It suppressed mRNA expression levels of MMP-1, MMP-3, hyaluronidase (HYAL)-1, HYAL-2, cyclooxygenase-2 (COX-2), interleukin (IL)-6, IL-8, and IL-1 beta in UVB-irradiated Hs68 fibroblasts. When Hs68 fibroblasts were exposed to hydrangenol after UVB irradiation, UVB-induced reactive oxygen species (ROS) production was suppressed. Hydrangenol also inhibited the activation of activator protein-1 (AP-1) and signal transduction and activation of transcription 1 (STAT-1) by downregulating phosphorylation of p38 and extracellular signal-regulated kinase (ERK). Our data indicate that hydrangenol isolated from H. serrata leaves has potential protective effects on UVB-induced skin photoaging. English PHARMACEUTICAL SOC JAPAN HYALURONIC-ACID SKIN MECHANISMS PHOSPHORYLATION SUPPRESSION GLUCOSIDES ACTIVATION EXTRACT Chemical Constituents from Leaves of Hydrangea serrata and Their Anti-photoaging Effects on UVB-Irradiated Human Fibroblasts Article 10.1248/bpb.b18-00742 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.42, no.3, pp.424 - 431 BIOLOGICAL & PHARMACEUTICAL BULLETIN 42 3 424 431 scie scopus 000460366200019 2-s2.0-85062405364 Pharmacology & Pharmacy Pharmacology & Pharmacy Article HYALURONIC-ACID SKIN MECHANISMS PHOSPHORYLATION SUPPRESSION GLUCOSIDES ACTIVATION EXTRACT Hydrangea serrata photoaging matrix metalloproteinase (MMP) procollagen type 1 skin fibroblast