Kim, Hyo Young Kang, Seong Jae Jeon, Yongmoon An, Jinsu Park, Jihyun Lee, Hee Jae Jang, Jeong-Eun Ahn, JongSeong Bang, Duhee Chung, Hak Suk Jeong, Cherlhyun Ahn, Dae-Ro 2024-01-19T20:33:25Z 2024-01-19T20:33:25Z 2021-09-02 2019-03 1359-7345 https://pubs.kist.re.kr/handle/201004/120291 We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA. English ROYAL SOC CHEMISTRY Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity Article 10.1039/c8cc08468h 1 CHEMICAL COMMUNICATIONS, v.55, no.24, pp.3552 - 3555 CHEMICAL COMMUNICATIONS 55 24 3552 3555 N scie scopus 000462654300025 2-s2.0-85063077532 Chemistry, Multidisciplinary Chemistry Article CRISPR RNA ENDONUCLEASE MUTATIONS NUCLEASES SYSTEM CRISPR Cas9 Chimeric crRNA target specificity