Yook, Sang Do Kim, Jiwon Woo, Han Min Um, Youngsoon Lee, Sun-Mi 2024-01-19T20:33:33Z 2024-01-19T20:33:33Z 2021-09-02 2019-03 0960-1481 https://pubs.kist.re.kr/handle/201004/120298 Biodiesel production using oleaginous yeasts, such as Yarrowia lipolytica, has recently attracted strong attention in terms of lipid yield and productivity. However, efficient methods of lipid extraction from oleaginous yeasts are required to further improve the economic feasibility of yeast-based biodiesel production. We investigated the effectiveness of selected switchable hydrophilicity solvents (SHSs), namely, N-ethylbutylamine (EB), N-dipropylamine (DP), and N,N-dimethylcyclohexyl-amine (DMCHA), for lipid extraction from the oleaginous yeast Y. lipolytica. Regardless of using harvested cells or culture broth, DMCHA and EB effectively extracted lipids from Y. lipolytica, resulting in up to 13% higher lipid yields than that obtained by conventional organic solvent extraction using a chloroform/methanol mixture. The lipid extracts obtained by the SHSs showed fatty acid compositions comparable to that obtained with the conventional solvent, thus assuring the quality of biodiesel produced through this method. After switching processes by purging with CO2 and N-2, the lipids and solvents were successfully recovered, demonstrating the practicability of lipid extraction using SHSs. Consequently, this study provides an effective lipid extraction method for Y. lipolytica that could significantly improve energy efficiency and economic feasibility of an oleaginous-yeast-based biodiesel production process. (C) 2018 Elsevier Ltd. All rights reserved. English Pergamon Press Ltd. Efficient lipid extraction from the oleaginous yeast Yarrowia lipolytica using switchable solvents Article 10.1016/j.renene.2018.07.129 1 Renewable Energy, v.132, pp.61 - 67 Renewable Energy 132 61 67 N scie scopus 000449892900006 2-s2.0-85053115777 Green & Sustainable Science & Technology Energy & Fuels Science & Technology - Other Topics Energy & Fuels Article BIODIESEL PRODUCTION HYDROPHILICITY SOLVENTS BIOFUEL PRODUCTION MICROALGAE BIOMASS MICROORGANISMS AMINES Switchable hydrophilicity solvents (SHSs) Yarrowia lipolytica Lipid extraction Biodiesel Microbial oil