Han, Boyoung Min, Hophil Jeon, Mijin Kang, Byeori Son, Junghyun 2024-01-19T20:33:39Z 2024-01-19T20:33:39Z 2021-09-02 2019-03 1942-7603 https://pubs.kist.re.kr/handle/201004/120304 Target analysis using liquid chromatography-tandem mass spectrometry is applied for rapidly detecting various prohibited doping substances. Frequent modification is required as additional substances are prohibited. We developed and validated a non-target screening method requiring no further modification because it analyzes the full spectrum of data in fixed m/z ranges. Urine samples were extracted using solid-phase extraction and analyzed by employing a method that combines full scan and variable data independent acquisition using high-resolution mass spectrometry; and all prohibited substances in the urine samples were successfully detected using our screening method. The method was validated in terms of specificity (no interferences), recoveries (29%-131%), matrix effects (35%-237%), limites of detection (0.0002-100 ng/mL), and intra- and inter-day precisions (coefficients of variation lower than 25%). The applicability of this method to doping tests was evaluated by analyzing 14 urine samples. As a result, the non-target screening method is efficient for conducting anti-doping tests because it can be applied without any further modification to prohibited drugs as well as to unknown targets that can be prohibited in the future. English WILEY DRUGS PERFORMANCE FIGHT MS/MS A rapid non-target screening method for determining prohibited substances in human urine using liquid chromatography/high-resolution tandem mass spectrometry Article 10.1002/dta.2495 1 DRUG TESTING AND ANALYSIS, v.11, no.3, pp.382 - 391 DRUG TESTING AND ANALYSIS 11 3 382 391 scie scopus 000462147600001 2-s2.0-85055534202 Biochemical Research Methods Chemistry, Analytical Pharmacology & Pharmacy Biochemistry & Molecular Biology Chemistry Pharmacology & Pharmacy Article DRUGS PERFORMANCE FIGHT MS/MS doping control high-resolution mass spectrometry Non-target screening method open method variable data independent acquisition