Kim, Jun-Sik Ahn, Jae-Pyeong Kim, Yang-Hee Seo, Kyung Won Zadeh, Homayoun Kim, Seong-Hun 2024-01-19T20:33:47Z 2024-01-19T20:33:47Z 2021-09-02 2019-03 0003-3219 https://pubs.kist.re.kr/handle/201004/120312 Objectives: To evaluate nanoscale molecular interactions in the interface between human bone and orthodontic titanium implants. Materials and Methods: An orthodontic implant (sandblasted with large grit and with an acid-etched surface treated with Ti6A14V alloy) retrieved from the mandible of human after 2 months of healing was used to analyze the molecular interactive mechanism between the implant and the surrounding bone tissue. To preserve the natural state of the sample as much as possible, cryofixation and scanning electron microscope/focused ion beam milling without any chemical treatment were used during sample preparation. Atom probe tomography was used to investigate the chemical composition and structure at the interface between the implant and human bone tissue. Results: Three-dimensional (3D) reconstruction of the whole sample revealed a 20 x 50-nm(2) plate-like bony element diffusion layer in the sample. The iso concentration analysis of the diffusion layer indicated that the bony element, calcium, and the implant element, titanium oxide, were interspersed with each other. Detailed ionic distribution was illustrated by 3D reconstruction with partial region of interest and one-dimensional concentration profiles of the implant-bone interface. Conclusions: The study results advance nanoscale understanding of osseointegration and suggest a potential nanostructure for increasing bond strength of biomaterials to bone. English E H ANGLE EDUCATION RESEARCH FOUNDATION, INC PROBE TOMOGRAPHY INTERFACE Atomic layout of an orthodontic titanium mini-implant in human tissue: Insights into the possible mechanisms during osseointegration Article 10.2319/051418-354.1 1 ANGLE ORTHODONTIST, v.89, no.2, pp.292 - 298 ANGLE ORTHODONTIST 89 2 292 298 scie scopus 000459005400015 2-s2.0-85061860372 Dentistry, Oral Surgery & Medicine Dentistry, Oral Surgery & Medicine Article PROBE TOMOGRAPHY INTERFACE Osseointegration Titanium implant Cryofixation Atom probe tomography Implant-bone interface SLA