Lee, Ae-Ree Hwang, Jihyun Hur, Jeong Hwan Ryu, Kyoung-Seok Kim, Kyeong Kyu Choi, Byong-Seok Kim, Nak-Kyoon Lee, Joon-Hwa 2024-01-19T21:01:13Z 2024-01-19T21:01:13Z 2021-09-02 2019-02 1554-8929 https://pubs.kist.re.kr/handle/201004/120414 Human RNA editing enzyme ADAR1 deaminates adenosine in pre-mRNA to yield inosine. The Z alpha domain of human ADAR1 (hZ alpha(ADAR1)) binds specifically to left-handed Z-RNA as well as Z-DNA and stabilizes the Z-conformation. To answer the question of how hZ alpha(ADAR1) can induce both the B-Z transition of DNA and the A-Z transition of RNA, we investigated the structure and dynamics of hZ alpha(ADAR1) in complex with 6-base-pair Z-DNA or Z-RNA. We performed chemical shift perturbation and relaxation dispersion experiments on hZ alpha(ADAR1) upon binding to Z-DNA as well as Z-RNA. Our study demonstrates the unique dynamics of hZ alpha(ADAR1) during the A-Z transition of RNA, in which the hZ alpha(ADAR1) protein forms a thermodynamically stable complex with Z-RNA, similar to Z-DNA, but kinetically converts RNA to the Z-form more slowly than DNA. We also discovered some distinct structural features of hZ alpha(ADAR1) in the Z-RNA binding conformation. Our results suggest that the A-Z transition of RNA facilitated by hZ alpha(ADAR1) displays unique structural and dynamic features that may be involved in targeting ADAR1 for a role in recognition of RNA substrates. English AMER CHEMICAL SOC Z-ALPHA DOMAIN B-Z TRANSITION NUCLEAR-MAGNETIC-RESONANCE HUMAN EDITING ENZYME HANDED Z-DNA BINDING DOMAIN ADENOSINE-DEAMINASE PROTEIN-KINASE CRYSTAL-STRUCTURE COMPLEX NMR Dynamics Study Reveals the Za Domain of Human ADAR1 Associates with and Dissociates from Z-RNA More Slowly than Z-DNA Article 10.1021/acschembio.8b00914 1 ACS CHEMICAL BIOLOGY, v.14, no.2, pp.245 - 255 ACS CHEMICAL BIOLOGY 14 2 245 255 scie scopus 000459367200013 2-s2.0-85060024504 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article Z-ALPHA DOMAIN B-Z TRANSITION NUCLEAR-MAGNETIC-RESONANCE HUMAN EDITING ENZYME HANDED Z-DNA BINDING DOMAIN ADENOSINE-DEAMINASE PROTEIN-KINASE CRYSTAL-STRUCTURE COMPLEX