Ryu, Jae Ryun Kim, June Hoan Cho, Hyo Min Jo, Youhwa Lee, Boram Joo, Sunghoon Chae, Uikyu Nam, Yoonkey Cho, Il-Joo Sun, Woong 2024-01-19T21:01:58Z 2024-01-19T21:01:58Z 2021-09-02 2019-01-21 1473-0197 https://pubs.kist.re.kr/handle/201004/120456 Neurons reach their correct targets by directional outgrowth of axons, which is mediated by attractive or repulsive cues. Growing axons occasionally cross a field of repulsive cues and stop at intermediate targets on the journey to their final destination. However, it is not well-understood how individual growth cones make decisions, and pass through repulsive territory to reach their permissive target regions. We developed a microcontact printing culture system that could trap individual axonal tips in a permissive dot area surrounded by the repulsive signal, semaphorin 3F (Sema3F). Axons of rat hippocampal neurons on the Sema3F/PLL dot array extended in the checkboard pattern with a significantly slow growth rate. The detailed analysis of the behaviors of axonal growth cones revealed the saccadic dynamics in the dot array system. The trapped axonal tips in the permissive area underwent growth cone enlargement with remarkably spiky filopodia, promoting their escape from the Sema3F constraints with straight extension of axons. This structured axonal growth on the dot pattern was disrupted by increased inter-dot distance, or perturbing intracellular signaling machineries. These data indicate that axons grow against repulsive signals by jumping over the repulsive cues, depending on contact signals and intracellular milieu. Our study suggests that our dot array culture system can be used as a screening system to easily and efficiently evaluate ECM or small molecule inhibitors interfering growth cone dynamics leading to controlling axonal growth. English Royal Society of Chemistry CONE BEHAVIOR MEMBRANE-LIPIDS GUIDANCE SLIT QUANTIFICATION LIPOPROTEINS NEUROPILIN-2 ELONGATION DROSOPHILA FILOPODIA A monitoring system for axonal growth dynamics using micropatterns of permissive and Semaphorin 3F chemorepulsive signals Article 10.1039/c8lc00845k 1 Lab on a Chip, v.19, no.2, pp.291 - 305 Lab on a Chip 19 2 291 305 scie scopus 000457299300007 2-s2.0-85060047071 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article CONE BEHAVIOR MEMBRANE-LIPIDS GUIDANCE SLIT QUANTIFICATION LIPOPROTEINS NEUROPILIN-2 ELONGATION DROSOPHILA FILOPODIA