Yoo, Wan Ki Ryu, Bum Han Kim, Kyoung-Ran Wang, Ying Ly Thi Huong Luu Le Lee, Jun Hyuck Kim, Kyeong Kyu Toth, Gergely Ahn, Dae-Ro Kim, T. Doohun 2024-01-19T21:02:58Z 2024-01-19T21:02:58Z 2021-09-05 2019-01 0304-4165 https://pubs.kist.re.kr/handle/201004/120510 The small presynaptic protein alpha-synuclein (alpha-syn) is involved in the etiology of Parkinson's disease owing to its abnormal misfolding. To date, little information is known on the role of DNA nanostructures in the formation of alpha-syn amyloid fibrils. Here, the effects of DNA tetrahedrons on the formation of alpha-syn amyloid fibrils were investigated using various biochemical and biophysical methods such as thioflavin T fluorescence assay, atomic force microscopy, light scattering, transmission electron microscopy, and cell-based cytotoxicity assay. It has been shown that DNA tetrahedrons decreased the level of oligomers and increased the level of amyloid fibrils, which corresponded to decreased cellular toxicity. The ability of DNA tetrahedron to facilitate the formation of alpha-syn amyloid fibrils demonstrated that structured nucleic acids such as DNA tetrahedrons could modulate the process of amyloid fibril formation. Our study suggests that DNA tetrahedrons could be used as an important facilitator toward amyloid fibril formation of alpha-synuclein, which may be of significance in finding therapeutic approaches to Parkinson's disease and related synucleinopathies. English ELSEVIER SCIENCE BV AMYLOID FORMATION PROTEIN AGGREGATION MECHANISM PEPTIDE Modulating alpha-synuclein fibril formation using DNA tetrahedron nanostructures Article 10.1016/j.bbagen.2018.09.025 1 BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v.1863, no.1, pp.73 - 81 BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1863 1 73 81 scie scopus 000452343100008 2-s2.0-85054424873 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article AMYLOID FORMATION PROTEIN AGGREGATION MECHANISM PEPTIDE Alpha-synuclein DNA tetrahedron Amyloid Parkinson&apos s disease