Zhao, Dong Gu, Ming-Yao Xu, Jiu Liang Zhang, Li Jun Ryu, Shi Yong Yang, Hyun Ok 2024-01-19T21:03:17Z 2024-01-19T21:03:17Z 2021-09-05 2019-01 1976-9148 https://pubs.kist.re.kr/handle/201004/120528 Ginger, one of worldwide consumed dietary spice, is not only famous as food supplements, but also believed to exert a variety of remarkable pharmacological activity as herbal remedies. In this study, a ginger constituent, 12-dehydrogingerdione (DHGD) was proven that has comparable anti-inflammatory activity with positive control 6-shogaol in inhibiting LPS-induced interleukin (IL)-6, tumor necrosis factor (TNF)-alpha, prostaglandin (PG) E-2, nitric oxide (NO), inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, without interfering with COX-1 in cultured microglial cells. Subsequent mechanistic studies indicate that 12-DHGD may inhibit neuro-inflammation through suppressing the LPS-activated Akt/IKK/NF-kappa B pathway. Furthermore, 12-DHGD markedly promoted the activation of NF-E2-related factor (Nrf)-2 and heme oxygenase (HO)-1, and we demonstrated that the involvement of HO-1 on the production of pro-inflammatory mediators such as NO and TNF-alpha by using a HO-1 inhibitor, Zinc protoporphyrin (Znpp). These results indicate that 12-DHGD may protect against neuro-inflammation by inhibiting Akt/IKK/kB/NF-kappa B pathway and promoting Nrf-2/HO-1 pathway. English KOREAN SOC APPLIED PHARMACOLOGY IKK-BETA 6-SHOGAOL GINGER INFLAMMATION ANTIOXIDANT EXPRESSION PI3K/AKT PHOSPHORYLATION MECHANISMS ELEMENTS Anti-neuroinflammatory Effects of 12-Dehydrogingerdione in LPS-Activated Microglia through Inhibiting Akt/IKK/NF-kappa B Pathway and Activating Nrf-2/HO-1 Pathway Article 10.4062/biomolther.2018.104 1 BIOMOLECULES & THERAPEUTICS, v.27, no.1, pp.92 - 100 BIOMOLECULES & THERAPEUTICS 27 1 92 100 scie scopus kci ART002414694 000455986100012 2-s2.0-85060706171 Biochemistry & Molecular Biology Pharmacology & Pharmacy Biochemistry & Molecular Biology Pharmacology & Pharmacy Article IKK-BETA 6-SHOGAOL GINGER INFLAMMATION ANTIOXIDANT EXPRESSION PI3K/AKT PHOSPHORYLATION MECHANISMS ELEMENTS Ginger 12-Dehydrogingerdione Neuro-inflammation Microglial cells Signaling pathway