Moon, Ju-Yeon Lee, Hye Suk Kim, Jun Ho Lee, Jin Hyup Choi, Man Ho 2024-01-19T21:04:09Z 2024-01-19T21:04:09Z 2021-09-05 2018-12-11 0003-2670 https://pubs.kist.re.kr/handle/201004/120576 Although, steroid profiling is being applied in clinical and biochemical studies, improvement of the technique is still needed for accurate quantification of steroids in case of limited biological sample volumes. To improve analytical sensitivity and selectivity in comparison to that of conventional methods, a method that comprises supported liquid extraction (SLE) and gas chromatography-mass spectrometry with a combination of selected-reaction and selected-ion monitoring modes (GC-SRM/SIM-MS) was developed. Here, this combination of SLE purification with GC-MS method was optimized with 37 different types of steroids and the results were compared to a solid-phase extraction (SPE) method. The devised assay led to an increase in extraction efficiency with the good chromatographic selectivity through a single extraction step. The limits of quantification of the serum steroids, ranged from 0.2 to 5 ngmL(-1), except for cholesterol (0.2 mgmL(-1)), and the correlation coefficients for calibration curves were higher than 0.99. The precision and accuracy were 1.4%-10.5% and 82.7%-115.3%, respectively. The overall recoveries of 30 steroids ranged from 62.1% to 104.3%, while that of 7 sterols was 44.7%-75.7%. Then, this validated method was applied to monitor the serum steroid levels of mice, which showed significant sex and age dependent metabolic patterns. This technique can be used to evaluate the metabolic changes occurring in animal models as well as in clinical patients. (C) 2018 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV LC-MS/MS SEX-DIFFERENCES MALE-MICE PLASMA CHOLESTEROL DISEASES HORMONES MOUSE PERFORMANCE METABOLITES Supported liquid extraction coupled to gas chromatography-selective mass spectrometric scan modes for serum steroid profiling Article 10.1016/j.aca.2018.02.059 1 ANALYTICA CHIMICA ACTA, v.1037, pp.281 - 292 ANALYTICA CHIMICA ACTA 1037 281 292 scie scopus 000446225000029 2-s2.0-85042872497 Chemistry, Analytical Chemistry Article LC-MS/MS SEX-DIFFERENCES MALE-MICE PLASMA CHOLESTEROL DISEASES HORMONES MOUSE PERFORMANCE METABOLITES Steroids SLE GC-MS Serum Mouse