Roh, Donghyeon Choi, Woongsun Kim, Junbeom Yu, Hyun-Yong Choi, Nakwon Cho, Il-Joo 2024-01-19T21:05:03Z 2024-01-19T21:05:03Z 2021-09-05 2018-12 1976-0280 https://pubs.kist.re.kr/handle/201004/120625 Hydrogel scaffolds composed of multiple components are promising platform in tissue engineering as a transplantation materials or artificial organs. Here, we present a new fabrication method for implementing multi-layered macroscopic hydrogel scaffold composed of multiple components by controlling height of hydrogel layer through precise control of ultraviolet (UV) energy density. Through the repetition of the photolithography process with energy control, we can form several layers of hydrogel with different height. We characterized UV energy-dependent profiles with single-layered PEGDA posts photocrosslinked by the modular methodology and examined the optical effect on the fabrication of multi-layered, macroscopic hydrogel structure. Finally, we successfully demonstrated the potential applicability of our approach by fabricating various macroscopic hydrogel constructs composed of multiple hydrogel layers. English KOREAN BIOCHIP SOCIETY-KBCS 3D BIOFABRICATION LITHOGRAPHY Fabrication of Multi-layered Macroscopic Hydrogel Scaffold Composed of Multiple Components by Precise Control of UV Energy Article 10.1007/s13206-018-2403-0 1 BIOCHIP JOURNAL, v.12, no.4, pp.280 - 286 BIOCHIP JOURNAL 12 4 280 286 scie scopus kci ART002413391 000453682800003 2-s2.0-85058652944 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article 3D BIOFABRICATION LITHOGRAPHY Photocrosslinking Macroscopic hydrogel scaffold Height control via UV energy density Multilayered structures Incorporation of multiple components Tissue engineering Artificial tissues organs