Kang, Dong-Hyun Han, Won Bae Choi, Nakwon Kirn, Yong-Jun Kim, Tae Song 2024-01-19T21:30:34Z 2024-01-19T21:30:34Z 2021-09-05 2018-11-28 1944-8244 https://pubs.kist.re.kr/handle/201004/120663 Artificial lipid membranes are excellent candidates for new biosensing platforms because their structures are similar to cell membranes and it is relatively easy to modify the composition of the membrane. The freestanding structure is preferable for this purpose because of the more manageable reconstitution of the membrane protein. Therefore, most of the lipid membranes for biosensing are based on two-dimensional structures that are fixed on a solid substrate (unlike floating liposomes) even though they have some disadvantages, such as low stability, small surface area, and potential retention of solvent in the membrane. In this paper, three-dimensional freestanding lipid bilayer (3D FLB) arrays were fabricated uniformly on SU-8 microwells without any toxic solvent. The 3D FLBs have better stability and larger surface area due to their cell-like structure. In order to improve the sealing characteristics of the 3D FLBs, the applied frequency of the ac field was controlled during the electroformation. The 3D FLBs were observed through transparent SU-8 microwell arrays using confocal microscopy and demonstrated perfect sealing until 5.5 days after the electroformation at more than 1 kHz. Also, the details of the sealing of a fixed 3D freestanding lipid structure were discussed for the first time. The unilamellarity and biofunctionality of the 3D FLBs were verified by a transport protein (alpha-hemolysin) assay. English American Chemical Society GIANT VESICLES CONTROLLED SIZE ELECTROFORMATION LIPOSOMES HYDRATION BILAYERS WATER Tightly Sealed 3D Lipid Structure Monolithically Generated on Transparent SU-8 Microwell Arrays for Biosensor Applications Article 10.1021/acsami.8b13458 1 ACS Applied Materials & Interfaces, v.10, no.47, pp.40401 - 40410 ACS Applied Materials & Interfaces 10 47 40401 40410 scie scopus 000451932800006 2-s2.0-85056814660 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article GIANT VESICLES CONTROLLED SIZE ELECTROFORMATION LIPOSOMES HYDRATION BILAYERS WATER artificial cell membrane 3D lipid bilayer structure freestanding lipid membrane sealing microwell arrays