Kim, Shin-Hye On, Ji-won Pyo, Heesoo Ko, Kyung Soo Won, Jong Chul Yang, Jiyeon Park, Mi Jung 2024-01-19T21:30:38Z 2024-01-19T21:30:38Z 2021-09-05 2018-11-27 1932-6203 https://pubs.kist.re.kr/handle/201004/120666 Objective We assessed the associations of percentage fractions of urinary di(2-ethylhexyl) phthalate (DEHP) metabolites with obesity and insulin resistance in Korean girls. Methods In total, 137 girls, aged 6 to 13 years (65 overweight cases and 72 controls), were recruited. Anthropometric indices and the homeostatic model assessment of insulin resistance (HOMA-IR) index were determined. Four major urinary DEHP metabolites were analyzed in spot urine samples by gas chromatography-tandem mass spectrometry, including mono(2-ethylhexyl) phthalate, mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), and mono(2-ethyl-5-carboxypentyl) phthalate. Results There were no significant differences in the urinary concentrations of the DEHP metabolites between the overweight and control groups. The percentage fraction of MEHHP (MEHHP %) among all DEHP metabolites was significantly higher in the overweight prepubertal girls than in the controls (P = 0.035). MEHHP% was positively associated with the body mass index percentile, waist circumference, body fat percentage, and HOMA-IR index in the prepubertal girls. After adjusting for covariates, the prepubertal girls in a higher MEHHP% quartile were found to have a higher odds ratio for central obesity than those in a lower quartile (odds ratios: 5.05 for quartile 3; 7.30 for quartile 4). The relative rate of MEHHP oxidation to MEOHP was negatively associated with the body mass index percentile and waist circumference in the prepubertal girls. However, no such association was observed in the pubertal girls. Conclusions MEHHP% was positively associated with obesity and insulin resistance in prepubertal girls. Further studies are necessary to elucidate the causal links between altered phthalate metabolism and increased susceptibility to insulin resistance in children. English PUBLIC LIBRARY SCIENCE WAIST CIRCUMFERENCE SPECIES-DIFFERENCES BODY-MASS DEHP EXPRESSION CHILDHOOD EXPOSURE ESTROGEN PATTERN GLUCOSE Percentage fractions of urinary di(2-ethylhexyl) phthalate metabolites: Association with obesity and insulin resistance in Korean girls Article 10.1371/journal.pone.0208081 1 PLOS ONE, v.13, no.11 PLOS ONE 13 11 scie scopus 000451440000041 2-s2.0-85057290480 Multidisciplinary Sciences Science & Technology - Other Topics Article WAIST CIRCUMFERENCE SPECIES-DIFFERENCES BODY-MASS DEHP EXPRESSION CHILDHOOD EXPOSURE ESTROGEN PATTERN GLUCOSE