Choi, Jae Hyouk Yarishkin, Oleg Kim, Eunju Bae, Yeonju Kim, Ajung Kim, Seung-Chan Ryoo, Kanghyun Choi, Chang-Hoon Hwang, Eun Mi Park, Jae-Yong 2024-01-19T21:30:57Z 2024-01-19T21:30:57Z 2022-01-10 2018-11-12 1226-3613 https://pubs.kist.re.kr/handle/201004/120684 Two-pore domain K+ (K2P) channels have been shown to modulate neuronal excitability. The physiological role of TWIK-1, the first identified K2P channel, in neuronal cells is largely unknown, and we reported previously that TWIK-1 contributes to the intrinsic excitability of dentate gyrus granule cells (DGGCs) in mice. In the present study, we investigated the coexpression of TWIK-1 and TASK-3, another K2P member, in DGGCs. Immunohistochemical staining data showed that TASK-3 proteins were highly localized in the proximal dendrites and soma of DGGCs, and this localization is similar to the expression pattern of TWIK-1. TWIK-1 was shown to associate with TASK-3 in DGGCs of mouse hippocampus and when both genes were overexpressed in COS-7 cells. shRNA-mediated gene silencing demonstrated that TWIK-1/TASK-3 heterodimeric channels displayed outwardly rectifying currents and contributed to the intrinsic excitability of DGGCs. Neurotensin-neurotensin receptor 1 (NT-NTSR1) signaling triggered the depolarization of DGGCs by inhibiting TWIK-1/TASK-3 heterodimeric channels, causing facilitated excitation of DGGCs. Taken together, our study clearly showed that TWIK-1/TASK-3 heterodimeric channels contribute to the intrinsic excitability of DGGCs and that their activities are regulated by NT-NTSR1 signaling. English 생화학분자생물학회 TWIK-1 K+ CHANNEL FUNCTIONAL HETERODIMERS SUBUNITS TASK-3 K2P1 EXCITABILITY EXPRESSION RECEPTORS SUBICULUM PROTEINS TWIK-1/TASK-3 heterodimeric channels contribute to the neurotensin-mediated excitation of hippocampal dentate gyrus granule cells Article 10.1038/s12276-018-0172-4 1 Experimental & Molecular Medicine, v.50 Experimental & Molecular Medicine 50 scie scopus kci ART002405423 000449910800001 2-s2.0-85056334515 Biochemistry & Molecular Biology Medicine, Research & Experimental Biochemistry & Molecular Biology Research & Experimental Medicine Article TWIK-1 K+ CHANNEL FUNCTIONAL HETERODIMERS SUBUNITS TASK-3 K2P1 EXCITABILITY EXPRESSION RECEPTORS SUBICULUM PROTEINS