Sung, Mi Sook Jung, Jik-Han Jeong, Cherlhyun Yoon, Tae-Young Park, Ji-Ho 2024-01-19T21:32:44Z 2024-01-19T21:32:44Z 2021-09-05 2018-10-18 1613-6810 https://pubs.kist.re.kr/handle/201004/120781 Cancer cells actively release extracellular vesicles (EVs) as important carriers of cellular information to tumor microenvironments. Although the composition and quantity of the proteins contained in EVs are characterized, it remains unknown how these proteins in EVs are related to those in the original cells at the functional level. With epidermal growth factor receptor (EGFR) in lung adenocarcinoma cells as a model oncoprotein, it is studied how distinct types of EVs, microvesicles and exosomes, represent their original cells at the protein and protein-protein interaction (PPI) level. Using the recently developed single-molecule immunolabeling and co-immunoprecipitation schemes, the quantity and PPI strengths of EGFRs derived from EVs and the original lung adenocarcinoma cells are determined. It is found that the microvesicles exhibit higher correlations with the original cells than the exosomes in terms of the EGFR levels and their PPI patterns. In spite of these detailed differences between the microvesicles and exosomes, the EGFR PPI strengths measured for EVs generally show a tight correlation with those determined for the original cells. The results suggest that EGFRs contained in EVs closely reflect the cellular EGFR in terms of their downstream signaling capacity. English WILEY-V C H VERLAG GMBH EXOSOMES MICROVESICLES CANCER BIOGENESIS MUTATIONS RELEASE Single-Molecule Co-Immunoprecipitation Reveals Functional Inheritance of EGFRs in Extracellular Vesicles Article 10.1002/smll.201802358 1 SMALL, v.14, no.42 SMALL 14 42 scie scopus 000447660700008 2-s2.0-85053667812 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article EXOSOMES MICROVESICLES CANCER BIOGENESIS MUTATIONS RELEASE cancer cell epidermal growth factor receptor extracellular vesicle protein-protein interaction signaling