Jo, Ala Sung, June Lee, Sanghee Nam, Hyunsung Lee, Hyo Won Park, Jongmin Kim, Hwan Myung Kim, Eunha Park, Seung Bum 2024-01-19T21:33:37Z 2024-01-19T21:33:37Z 2021-09-05 2018-10 1043-1802 https://pubs.kist.re.kr/handle/201004/120828 Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with D/L-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells. English AMER CHEMICAL SOC POSITRON-EMISSION-TOMOGRAPHY CANCER-CELLS IN-VIVO TRANSPORT PROTEINS ANALOG FLUOROPHORE DYES GLUT RED Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells Article 10.1021/acs.bioconjchem.8b00558 1 BIOCONJUGATE CHEMISTRY, v.29, no.10, pp.3394 - 3401 BIOCONJUGATE CHEMISTRY 29 10 3394 3401 scie scopus 000447953500017 2-s2.0-85053601394 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article POSITRON-EMISSION-TOMOGRAPHY CANCER-CELLS IN-VIVO TRANSPORT PROTEINS ANALOG FLUOROPHORE DYES GLUT RED