Shafiq, Muhammad Zhang, Yue Zhu, Dashuai Zhao, Zongxian Kim, Dong-Hwee Kim, Soo Hyun Kong, Deling 2024-01-19T21:33:57Z 2024-01-19T21:33:57Z 2021-09-05 2018-10 2056-3418 https://pubs.kist.re.kr/handle/201004/120847 Cardiovascular diseases cause huge socio-economic burden worldwide. Although a mammalian myocardium has its own limited healing capability, scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium. The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P (SP) and insulin-like growth factor-1C (IGF-1C) peptide. Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning, which exhibited nanofibrous morphology. SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro. The developed patches were transplanted in an infarcted myocardium for up to 14 days. Mice underwent left anterior descending artery ligation and received one of the following treatments: (i) sham, (ii) saline, (iii) patch-only, (iv) IGF-1C patch, (v) SP patch and (vi) IGF-1C/SP patch. SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline, patch-only and individual peptide containing cardiac patches. SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups. IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups. Moreover, IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups. These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair. English OXFORD UNIV PRESS STEM-CELL RECRUITMENT ACUTE MYOCARDIAL-INFARCTION TISSUE REGENERATION C-DOMAIN ANGIOGENESIS ACTIVATION THERAPY IMPROVES DELIVERY REPAIR In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches Article 10.1093/rb/rby021 1 REGENERATIVE BIOMATERIALS, v.5, no.5, pp.303 - 316 REGENERATIVE BIOMATERIALS 5 5 303 316 scie scopus 000453585100006 2-s2.0-85073764097 Materials Science, Biomaterials Materials Science Article STEM-CELL RECRUITMENT ACUTE MYOCARDIAL-INFARCTION TISSUE REGENERATION C-DOMAIN ANGIOGENESIS ACTIVATION THERAPY IMPROVES DELIVERY REPAIR cardiac patch stem cell recruitment myocardial infarction electrospinning