Lee, Sang-Bin Kim, Hee-Tae Yang, Hyun Ok Jang, Wooyoung 2024-01-19T21:34:03Z 2024-01-19T21:34:03Z 2021-09-05 2018-10 2045-2322 https://pubs.kist.re.kr/handle/201004/120852 Parkinson's disease (PD) is a neurodegenerative disorder characterized by the accumulation of protein inclusions and the loss of dopaminergic neurons. Transcranial direct current stimulation (tDCS) is a non-invasive brain-stimulating technique that has demonstrated promising results in clinical studies of PD. Despite accumulating evidence indicating that tDCS exerts a protective effect, the mechanism underlying its activity remains unknown. In the present study, we first investigated the neuroprotective effect of tDCS in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model and then evaluated the effect of tDCS on the autophagy pathway. tDCS improved behavioral alterations, increased tyrosine hydroxylase protein levels and suppressed alpha-synuclein protein levels in MPTP-treated mice. MPTP-treated mice subjected to tDCS also had lower levels of autophagy-related proteins, such as microtubule-associated protein 1 light chain 3 and AMP-activated protein kinase, and higher levels of mechanistic target of rapamycin and p62. In addition, the protein levels of phosphoinositide 3-kinase and brain-derived neurotrophic factor were higher, and the levels of unc51-like kinase 1 were lower in MPTP-treated mice subjected to tDCS. Our findings suggest that tDCS protected against MPTP-induced PD in a mouse model by modulating autophagy. English Nature Publishing Group Anodal transcranial direct current stimulation prevents methyl-4phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity by modulating autophagy in an in vivo mouse model of Parkinson's disease Article 10.1038/s41598-018-33515-7 1 Scientific Reports, v.8 Scientific Reports 8 Y scie scopus 000447083100056 2-s2.0-85054777772 Multidisciplinary Sciences Science & Technology - Other Topics Article CHAPERONE-MEDIATED AUTOPHAGY ALPHA-SYNUCLEIN MPTP TDCS MECHANISMS DEFICITS STRESS MEMORY