Suhaeri, Muhammad Noh, Mi Hee Moon, Ji-Hoi Kim, In Gul Oh, Seung Ja Ha, Sang Su Lee, Jong Ho Park, Kwideok 2024-01-19T21:34:33Z 2024-01-19T21:34:33Z 2021-09-03 2018-10 1838-7640 https://pubs.kist.re.kr/handle/201004/120879 Skin injuries are frequently encountered in daily life, but deep wounds often poorly self-heal and do not recover completely. In this study, we propose a novel skin patch that combines antibiotic, cell-derived extracellular matrix (ECM) and biocompatible polyvinyl alcohol (PVA) hydrogel. Methods: Decellularized human lung fibroblast-derived matrix (hFDM) was prepared on tissue culture plate (TCP) and PVA solution was then poured onto it. After a freeze-thaw process, PVA was peeled off from TCP along with hFDM tightly anchored to PVA. Subsequently, ciprofloxacin (Cipro)-incorporated PVA/hFDM (PVA/Cipro/hFDM) was fabricated via diffusion-based drug loading. Results: In vitro analyses of PVA/Cipro/hFDM show little cytotoxicity of ciprofloxacin, stability of hFDM, rich fibronectin in hFDM, and good cell attachment, respectively. In addition, hFDM proved to be beneficial in promoting cell migration of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs) using transwell inserts. The antibacterial drug Cipro was very effective in suppressing colony growth of gram-negative and -positive bacteria as identified via an inhibition zone assay. For animal study, infected wound models in BALB/c mice were prepared and four test groups (control, PVA, PVA/Cipro, PVA/Cipro/hFDM) were administered separately and their effect on wound healing was examined for up to 21 days. The results support that Cipro successfully reduced bacterial infection and thus encouraged faster wound closure. Further analysis using histology and immunofluorescence revealed that the most advanced skin regeneration was achieved with PVA/Cipro/hFDM, as assessed via re-epithelialization, collagen texture and distribution in the epidermis, and skin adnexa (i.e., glands and hair follicles) regeneration in the dermis. Conclusion: This work demonstrates that our skin patch successfully consolidates the regenerative potential of ECM and the antibacterial activity of Cipro for advanced wound healing. English IVYSPRING INT PUBL POLY(VINYL ALCOHOL) HYDROGELS PRIMARY ENDOTHELIAL-CELLS EXTRACELLULAR-MATRIX VASCULAR MORPHOGENESIS CONTROLLED-RELEASE GROWTH-FACTORS KILL BACTERIA MICROENVIRONMENT DIFFERENTIATION REPAIR Novel skin patch combining human fibroblast-derived matrix and ciprofloxacin for infected wound healing Article 10.7150/thno.26837 1 THERANOSTICS, v.8, no.18, pp.5025 - 5038 THERANOSTICS 8 18 5025 5038 scie scopus 000447839500011 2-s2.0-85065349611 Medicine, Research & Experimental Research & Experimental Medicine Article POLY(VINYL ALCOHOL) HYDROGELS PRIMARY ENDOTHELIAL-CELLS EXTRACELLULAR-MATRIX VASCULAR MORPHOGENESIS CONTROLLED-RELEASE GROWTH-FACTORS KILL BACTERIA MICROENVIRONMENT DIFFERENTIATION REPAIR wound healing skin patch human fibroblast-derived matrix ciprofloxacin polyvinyl alcohol hydrogel