Seo, Youngmin Hwang, Jangsun Lee, Eunwon Kim, Young Jin Lee, Kyungwoo Park, Chanhwi Choi, Yonghyun Jeon, Hojeong Choi, Jonghoon 2024-01-19T22:00:36Z 2024-01-19T22:00:36Z 2021-09-03 2018-09 2040-3364 https://pubs.kist.re.kr/handle/201004/120921 Biofilms adhere to surfaces to produce extracellular polymeric substances (EPSs). EPSs grow and protect themselves from external stresses. Their formation causes a foul odor and may lead to chronic infectious diseases in animals and people. Biofilms also inhibit the contact between bacteria and antibiotics, thereby reducing their antibacterial activity. Thus, we describe novel nanostructures, a fusion of copper and multi-walled carbon nanotubes (MWCNTs), which increase antimicrobial activity against biofilms without being toxic to human cells. Simulations based on the stochastic response were performed to predict the efficiency of synthesizing nanostructures. The synthesized Cu/MWCNTs inhibit the growth of Methylobacterium spp., which forms biofilms; antimicrobial testing and cytotoxicity assessments showed that the Cu/MWCNTs were not cytotoxic to human cells. The Cu/MWCNTs come in direct contact with the bacterial cell surface, damage the cell wall, and cause secondary oxidation of reactive oxygen species. Furthermore, the Cu/MWCNTs release copper ions, which inhibit the quorum sensing in Methylobacterium spp., thereby inhibiting the expression of the genes that form biofilms. Additionally, we confirmed excellent electrical and thermal conductivity of Cu/MWCNTs as well as biofilm removal efficiency in the microfluidic channel. English Royal Society of Chemistry Engineering copper nanoparticles synthesized on the surface of carbon nanotubes for anti-microbial and anti-biofilm applications Article 10.1039/c8nr02768d 1 Nanoscale, v.10, no.33, pp.15529 - 15544 Nanoscale 10 33 15529 15544 N scie scopus 000443623800013 2-s2.0-85052500171 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Chemistry Science & Technology - Other Topics Materials Science Physics Article QUORUM-SENSING SIGNALS SODIUM DODECYL-SULFATE SILVER NANOPARTICLES ESCHERICHIA-COLI ANTIBACTERIAL ACTIVITY OXIDE NANOPARTICLES METHYLOBACTERIUM-EXTORQUENS BACTERIAL BIOLUMINESCENCE SUPEROXIDE DISMUTASES THERMAL-CONDUCTIVITY cell-cell communication quorum-sensing signals sodium dodecyl-sulfate gram-negative bacteria silver nanoparticles escherichia-coli antibacterial activity oxide nanoparticles