김익태 김낙균 서정용 2024-01-19T22:01:14Z 2024-01-19T22:01:14Z 2021-09-06 2018-09 1226-6531 https://pubs.kist.re.kr/handle/201004/120951 The CRISPR-Cas system is the adaptive immune system in bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, an endonuclease Cas9 cleaves DNA targets of phages as directed by guide RNA comprising crRNA and tracrRNA. To avoid targeting and destruction by Cas9, phages employ anti-CRISPR (Acr) proteins that act against host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone 1H, 15N, and 13C resonance assignments of AcrIIA4 that inhibits endonuclease activity of type II-A Listeria monocytogenes Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The secondary structures of AcrIIA4 predicted by the backbone chemical shifts show an alpha-beta-beta-beta-alpha-alpha fold, which is used to determine the solution structure. English 한국자기공명학회 1H, 15N, and 13C Resonance Assignments of the Anti-CRISPR AcrIIA4 from Listeria monocytogenes Prophages Article 10.6564/JKMRS.2018.22.3.071 2 Journal of the Korean Magnetic Resonance Society, v.22, no.3, pp.71 - 75 Journal of the Korean Magnetic Resonance Society 22 3 71 75 kci ART002384442 AcrIIA4 anti-CRISPR Cas9 NMR spectroscopy