Chang, Hyun-Kyung Kim, Pyung-Hwan Kim, Dong Wook Cho, Hyun-Min Jeong, Mi Jin Kim, Dea Han Joung, Yoon Ki Lim, Kyung Seob Kim, Han Byul Lim, Han Cheol Han, Dong Keun Hong, Young Joon Cho, Je-Yoel 2024-01-19T22:01:17Z 2024-01-19T22:01:17Z 2021-09-03 2018-09 1226-3613 https://pubs.kist.re.kr/handle/201004/120954 Atherosclerotic plaques within the vasculature may eventually lead to heart failure. Currently, cardiac stenting is the most effective and least invasive approach to treat this disease. However, in-stent restenosis is a complex chronic side effect of stenting treatment. This study used coronary stents coated with stem cells secreting angiogenic growth factors via an inducible genome-editing system to reduce stent restenosis and induce re-endothelialization within the artery. The characteristics of the cells and their adhesion properties on the stents were confirmed, and the stents were transplanted into a swine model to evaluate restenosis and the potential therapeutic use of stents with stem cells. Restenosis was evaluated using optical coherence tomography (OCT), microcomputed tomography (mCT) and angiography, and re-endothelialization was evaluated by immunostaining after cardiac stent treatment. Compared to a bare metal stent (BMS) or a parental umbilical cord blood-derived mesenchymal stem cell (UCB-MSC)-coated stent, the stents with stem cells capable of the controlled release of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) successfully reduced restenosis within the stent and induced natural re-endothelialization. Furthermore, UCB-MSCs exhibited the ability to differentiate into endothelial cells in Matrigel, and HGF and VEGF improved this differentiation. Our study indicates that stents coated with UCB-MSCs secreting VEGF/HGF reduce the restenosis side effects of cardiac stenting with improved re-endothelialization. English 생화학분자생물학회 Coronary stents with inducible VEGF/HGF-secreting UCB-MSCs reduced restenosis and increased re-endothelialization in a swine model Article 10.1038/s12276-018-0143-9 1 Experimental & Molecular Medicine, v.50 Experimental & Molecular Medicine 50 N scie scopus kci 000443220300001 2-s2.0-85052677413 Biochemistry & Molecular Biology Medicine, Research & Experimental Biochemistry & Molecular Biology Research & Experimental Medicine Article MESENCHYMAL STEM-CELLS DRUG-ELUTING STENTS GROWTH-FACTOR EXTRACELLULAR-MATRIX PROGENITOR CELLS FIBROSIS RESOLUTION INDUCTION INJECTION DISEASE