Park, SungJun Ko, Young-Seon Lee, Su Jin Lee, Cheonghoon Woo, Kyoungja Ko, GwangPyo 2024-01-19T22:01:40Z 2024-01-19T22:01:40Z 2021-09-03 2018-09 0944-1344 https://pubs.kist.re.kr/handle/201004/120974 Influenza A virus (IFV-A) is one of the main cause of seasonal flu and can infect various of host species via the reassortment of segmented RNA genomes. Silver nanoparticles (AgNPs) have been known as excellent antiviral agent against IFV. However, the use of free AgNPs has several major drawbacks, including the inherent aggregation among AgNPs and unwanted cytotoxic or genotoxic damages for human body via inhalation or ingestion. In this study, we assessed the efficacy of our novel 30-nm-diameter AgNP-decorated silica hybrid composite (Ag30-SiO2; 400 nm in diameter) for IFV-A inactivation. Ag30-SiO2 particles can inhibit IFV-A effectively in a clear dose-dependent manner. However, when real-time RT-PCR assay was used, merely 0.5-log(10) reduction of IFV-A was observed at both 5 and 20 A degrees C. Moreover, even after 1 h of exposure to Ag30-SiO2 particles, more than 80% of hemagglutinin (HA) damage and 20% of neuraminidase (NA) activities had occurred, and the infection of Madin-Darby Canine Kidney (MDCK) cells by IFV-A was reduced. The results suggested that the major antiviral mechanism of Ag30-SiO2 particles is the interaction with viral components located at the membrane. Therefore, Ag30-SiO2 particles can cause nonspecific damage to various IFV-A components and be used as an effective method for inactivating IFV-A. English SPRINGER HEIDELBERG CHICKEN MEAT DISINFECTION CYTOTOXICITY ENVIRONMENTS REPLICATION NANOSILVER PARTICLES COLLOIDS CELLS Inactivation of influenza A virus via exposure to silver nanoparticle-decorated silica hybrid composites Article 10.1007/s11356-018-2620-z 1 ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, v.25, no.27, pp.27021 - 27030 ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 25 27 27021 27030 scie scopus 000444202800032 2-s2.0-85049960991 Environmental Sciences Environmental Sciences & Ecology Article CHICKEN MEAT DISINFECTION CYTOTOXICITY ENVIRONMENTS REPLICATION NANOSILVER PARTICLES COLLOIDS CELLS Silver nanoparticle Silica hybrid composite Influenza A virus Antiviral agent Disinfection Virus inactivation