Lee, Boyoung Bang, Eunyoung Yang, Won Suk Paydar, Afshin Ha, Go Eun Kim, Sujin Kim, Jong-Hyun Cho, Taesup Lee, Seung Eun Lee, Sukchan Kang, Myoung-Goo Cheong, Eunji Kim, Key-Sun Lee, Cheolju Yu, Myeong-Hee Shin, Hee-Sup 2024-01-19T22:01:45Z 2024-01-19T22:01:45Z 2021-09-03 2018-09 2045-2322 https://pubs.kist.re.kr/handle/201004/120979 Established fear memory becomes vulnerable to disruption after memory retrieval and extinction; this labile state is critical for inhibiting the return of fear memory. However, the labile state has a very narrow time window after retrieval, and underlying molecular mechanisms are not well known. To that end, we isolated the hippocampus immediately after fear memory retrieval and performed proteomics. We identified Neurobeachin (NBEA), an autism-related regulator of synaptic protein trafficking, to be upregulated after contextual fear memory retrieval. NBEA protein expression was rapid and transient after fear memory retrieval at the synapse. Nbea mRNA was enriched at the synapses, and the rapid induction of NBEA expression was blocked by inhibition of the mammalian target of rapamycin (mTOR)-dependent signaling pathway. Mice with cornu ammonis 1 (CA1)-specific Nbea shRNA knockdown showed normal fear acquisition and contextual fear memory but impaired extinction, suggesting an important role of Nbea in fear memory extinction processes. Consistently, Nbea heterozygotes showed normal fear acquisition and fear memory recall but showed impairment in extinction. Our data suggest that NBEA is necessary either for induction of memory lability or for the physiological process of memory extinction. English NATURE PUBLISHING GROUP The Possible Role of Neurobeachin in Extinction of Contextual Fear Memory Article 10.1038/s41598-018-30589-1 1 SCIENTIFIC REPORTS, v.8 SCIENTIFIC REPORTS 8 N scie scopus 000444501200032 2-s2.0-85053329980 Multidisciplinary Sciences Science & Technology - Other Topics Article PROTEIN-SYNTHESIS MAMMALIAN TARGET SYNAPTIC PLASTICITY RECEPTOR TRAFFICKING POSTTRAUMATIC-STRESS GLUTAMATE RECEPTORS RECONSOLIDATION RETRIEVAL RAPAMYCIN INHIBITION