Mok, Il-Kyoon Lee, Jae Kwon Kim, Jeong Hwa Pan, Cheol-Ho Kim, Sang Min 2024-01-19T22:02:26Z 2024-01-19T22:02:26Z 2021-08-31 2018-08-30 0308-8146 https://pubs.kist.re.kr/handle/201004/121017 Our previous study reported the improved stability of fucoxanthin (FX) fortified in whole milk (WM) and skimmed milk (SM). In this study, in vivo and in vitro FX bioavailability were investigated using FX-fortified milk (FX-SM and FX-WM) and microalga Phaeodactylum tricornutum biomass (Pt-powder). Organ tissue accumulation of FX and its metabolites (FXOH: fucoxanthinol, AXA: amarouciaxanthin A) after repeated oral administration was in the following order: FX-SM > FX-WM > Pt-powder. In vivo pharmacokinetic study with a single oral administration also demonstrated that the absorption of FXOH and AXA was the highest for FX-SM. To reinforce the in vivo results, in vitro-simulated digestion and Caco-2 cell uptake assays were performed, which revealed that FX-SM showed the highest FX bioaccessibility (release from food matrices) and cellular uptake efficiency of FX and FXOH. In conclusion, skimmed milk was validated as an excellent food matrix for FX application in terms of stability and bioavailability. English ELSEVIER SCI LTD CAROTENOIDS STABILITY BIOACCESSIBILITY ASTAXANTHIN METABOLITES ABSORPTION CELLS ACID Fucoxanthin bioavailability from fucoxanthin-fortified milk: In vivo and in vitro study Article 10.1016/j.foodchem.2018.03.047 1 FOOD CHEMISTRY, v.258, pp.79 - 86 FOOD CHEMISTRY 258 79 86 scie scopus 000430241500011 2-s2.0-85044122821 Chemistry, Applied Food Science & Technology Nutrition & Dietetics Chemistry Food Science & Technology Nutrition & Dietetics Article CAROTENOIDS STABILITY BIOACCESSIBILITY ASTAXANTHIN METABOLITES ABSORPTION CELLS ACID Bioavailability Bioaccessibility Fucoxanthin Skimmed milk Phaeodactylum tricornutum