Jeon, Yong-Joon Shin, Jong-Il Lee, Sol Lee, Yoon Gyeong Kim, Ji Beom Kwon, Hak Cheol Kim, Sung Hun Kim, Inki Lee, Kyungho Han, Ye Sun 2024-01-19T22:03:12Z 2024-01-19T22:03:12Z 2022-01-25 2018-08 2314-6133 https://pubs.kist.re.kr/handle/201004/121057 Natural products are valuable sources for drug discovery because they have a wide variety of useful chemical components and biological properties. A quick reevaluation of the potential therapeutic properties of established natural products was made possible by the recent development of the methodology and improvement in the accuracy of an automated high-throughput screening system. In this study, we screened natural product libraries to detect compounds with anticancer effects using HeLa cells. Of the 420 plant extracts screened, the extract of Angelica gigas Nakai (AGN) was the most effective in reducing cell viability of HeLa cells. Markers of apoptosis, such as exposure of phosphatidylserine and cleavage of caspase-7 and PARP, were increased by treatment with the AGN extract. Treatment of the AGN extract increased expression of PKR as well as ATF4 and CHOP, the unfolded protein response genes. In addition, cotreatment of doxorubicin and the AGN extract significantly increased doxorubicininduced apoptosis in HeLa cells. Decursin and decursinol angclate, which were known to have anticancer effects, were the main components of the AGN extract. These results suggest that the extract of AGN containing, decursin and decursinol angelate, increases doxorubicin susceptibility. English HINDAWI LTD Angelica gigas Nakai Has Synergetic Effects on Doxorubicin-Induced Apoptosis Article 10.1155/2018/6716547 1 BIOMED RESEARCH INTERNATIONAL, v.2018 BIOMED RESEARCH INTERNATIONAL 2018 N scie scopus 000441534500001 2-s2.0-85051583729 Biotechnology & Applied Microbiology Medicine, Research & Experimental Biotechnology & Applied Microbiology Research & Experimental Medicine Article ENDOPLASMIC-RETICULUM STRESS CHOP-INDUCED APOPTOSIS NATURAL-PRODUCTS DRUG DISCOVERY P-GLYCOPROTEIN CELL-DEATH CANCER DECURSIN CYCLOPHOSPHAMIDE CHEMOTHERAPY