Kim, Myung Goo Jo, Sung Duk Jeong, Ji Hoon Kim, Sun Hwa 2024-01-19T22:03:23Z 2024-01-19T22:03:23Z 2022-01-10 2018-08 1226-086X https://pubs.kist.re.kr/handle/201004/121067 To precisely regulate target genes that are abnormally expressed in cancers, we suggest an RNA-mediated multigene targeting system that co-encapsulates siRNA against vascular endothelial growth factor (VEGF) and mRNA encoding phosphatase and tensin homolog (PTEN). Polymerized long-chain siRNAs (L-siRNAs) formed stable and condensed nanocomplexes with mRNAs using thiolated glycol chitosans (tGCs) as gene carriers. The mRNA/L-siRNA/tGC nanocomplexes (MSNs) exhibited efficient intracellular delivery and superior anti-tumor efficiency with simultaneous up and down-regulation of PTEN and VEGF, respectively. The MSN system can be considered as a new platform for cancer gene therapy requiring accurate control of multiple gene expressions. (C) 2018 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. English 한국공업화학회 Nanoscale polyelectrolyte complexes encapsulating mRNA and long-chained siRNA for combinatorial cancer gene therapy Article 10.1016/j.jiec.2018.04.005 1 Journal of Industrial and Engineering Chemistry, v.64, pp.430 - 437 Journal of Industrial and Engineering Chemistry 64 430 437 N scie scopus kci ART002377718 000437068500044 2-s2.0-85046168531 Chemistry, Multidisciplinary Engineering, Chemical Chemistry Engineering Article GLYCOL CHITOSAN NANOPARTICLES DELIVERY-SYSTEMS PERSISTENCE LENGTH NONVIRAL VECTORS CELLULAR UPTAKE STRANDED RNA THERAPEUTICS STABILITY VEGF DNA Gene therapy siRNA mRNA Polyelectrolyte complex