Lee, Bo-Ram Jo, Eunji Yoon, Hong Yeol Yoon, Chul Joo Lee, Hyo-Jung Kwon, Koo Chul Kim, Tae Woo Lee, Jeewon 2024-01-19T22:03:36Z 2024-01-19T22:03:36Z 2021-09-03 2018-08 2198-3844 https://pubs.kist.re.kr/handle/201004/121079 Although protein nanoparticles (PNPs) (e.g., viral capsids) capable of delivering a broad range of drug agents have shown distinctive advantages over synthetic nanomaterials, PNPs have an intrinsic drawback that hampers their clinical application, that is, potential immunogenicity. Here, a novel method for resolving the immunogenicity problem of PNPs, which is based on the genetic presentation of albumin-binding peptides (ABPs) on the surface of PNP, is reported. ABPs are inserted into the surface of a viral capsid (hepatitis B virus capsid/HBVC) while preserving the native self-assembly function of HBVC. The ABPs effectively gather human serum albumins around HBVC and significantly reduce both inflammatory response and immunoglobulin titer in live mice compared to ABP-free HBVC. Furthermore, ABP-conjugated HBVCs remain within tumors for a longer period than HBVCs conjugated to tumor cell receptor-bindingpeptides, indicating that the ABPs are also capable of enhancing tumor-targeting performance. Although applied to HBVC for proof of concept, this novel approach may provide a general platform for resolving immunogenicity and cancer-targeting problems of PNPs, which enables the development of a variety of PNP-based drug delivery carriers with high safety and efficacy. English WILEY HUMAN SERUM-ALBUMIN DRUG-DELIVERY IN-VIVO MOSAIC-VIRUS NANOPARTICLES IMMUNOGENICITY PROTEIN SURFACE DESIGN SIRNA Nonimmunogenetic Viral Capsid Carrier with Cancer Targeting Activity Article 10.1002/advs.201800494 1 ADVANCED SCIENCE, v.5, no.8 ADVANCED SCIENCE 5 8 scie scopus 000441869400020 2-s2.0-85051562561 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article HUMAN SERUM-ALBUMIN DRUG-DELIVERY IN-VIVO MOSAIC-VIRUS NANOPARTICLES IMMUNOGENICITY PROTEIN SURFACE DESIGN SIRNA albumin albumin-binding peptides immunogenicity protein nanoparticles tumor targeting viral capsids