Choi, Minkyu Cho, Kyungjin Lee, Seockheon Chung, Yun-Chul Park, Joonhong Bae, Hyokwan 2024-01-19T22:04:02Z 2024-01-19T22:04:02Z 2021-09-03 2018-08 0045-6535 https://pubs.kist.re.kr/handle/201004/121104 In this study, anammox enrichment reactors were operated using flat type poly (vinyl alcohol) cryogel (cryoPVAG) with precultured anammox bacteria (PAB) and activated sludge (AS) from an anoxic tack of the A2O process to evaluate the effect of different seeding sources on anammox enrichment. In addition, cryoPVAGs with different thicknesses (1, 2, and 3 mm) were used to investigate the effects of the thickness on anammox enrichment. The regression analysis with a modified Gompertz model showed that the start-up period of the anammox enrichment using PAB inoculum was approximately 14 days earlier than that of AS inoculum at a nitrogen loading rate of approximately 1 kg-N m(-3) day(-1). Substrate diffusion was limited in 3-mm cryoPVAG with respect to trend in nitrogen removal rate. Quantitative PCR analysis indicated that in the initial phase, the 16S rRNA gene copy numbers of anammox microorganism in cryoPVAG were significantly different according to the seeding source, but finally converged to a similar level after anammox enrichment. The anammox reaction was initially promoted by cryoPVAG. Next, anammox biomass detached from cryoPVAG and enriched in the bulk phase to maximize NRR. Illumina MiSeq sequencing revealed that Candidatus Brocadia sinica led to the active anammox reaction, and its relative abundance decreased with increasing gel thickness. (C) 2018 Elsevier Ltd. All rights reserved. English Pergamon Press Ltd. Effective seeding strategy using flat type poly (vinyl alcohol) cryogel for anammox enrichment Article 10.1016/j.chemosphere.2018.04.055 1 Chemosphere, v.205, pp.88 - 97 Chemosphere 205 88 97 N scie scopus 000439640900012 2-s2.0-85046531231 Environmental Sciences Environmental Sciences & Ecology Article; Proceedings Paper ANAEROBIC AMMONIUM OXIDATION NITROGEN REMOVAL PERFORMANCE ACTIVATED-SLUDGE POLYVINYL-ALCOHOL START-UP IMPROVEMENT MICROORGANISMS BACTERIA WATER IMMOBILIZATION Anaerobic ammonium oxidation Poly (vinyl alcohol) cryogel Inoculum source Substrate diffusion limitation Illumina MiSeq