Chun, So Young Kim, Dae Hwan Kim, Jeong Shik Kim, Hyun Tae Yoo, Eun Sang Chung, Jae-Wook Ha, Yun-Sok Song, Phil Hyun Joung, Yoon Ki Han, Dong Keun Chung, Sung Kwang Kim, Bum Soo Kwon, Tae Gyun 2024-01-19T22:04:18Z 2024-01-19T22:04:18Z 2021-09-03 2018-08 1738-2696 https://pubs.kist.re.kr/handle/201004/121119 Kidney ischemia-reperfusion (IR) via laparotomy is a conventional method for kidney surgery in a mouse model. However, IR, an invasive procedure, can cause serious acute and chronic complications through apoptotic and inflammatory pathways. To avoid these adverse responses, a Non-IR and dorsal slit approach was designed for kidney surgery. Animals were divided into three groups, 1) sham-operated control; 2) IR, Kidney IR via laparotomy; and 3) Non-IR, Non-IR and dorsal slit. The effects of Non-IR method on renal surgery outcomes were verified with respect to animal viability, renal function, apoptosis, inflammation, fibrosis, renal regeneration, and systemic response using histology, immunohistochemistry, real-time polymerase chain reaction, serum chemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and Masson's trichrome staining. The Non-IR group showed 100% viability with mild elevation of serum blood urea nitrogen and creatinine values at day 1 after surgery, whereas the IR group showed 20% viability and lethal functional abnormality. Histologically, renal tubule epithelial cell injury was evident on day 1 in the IR group, and cellular apoptosis enhanced TUNEL-positive cell number and Fas/caspase-3 and KIM-1/NGAL expression. Inflammation and fibrosis were high in the IR group, with enhanced CD4/CD8-positive T cell infiltration, inflammatory cytokine secretion, and Masson's trichrome stain-positive cell numbers. The Non-IR group showed a suitable microenvironment for renal regeneration with enhanced host cell migration, reduced immune cell influx, and increased expression of renal differentiation-related genes and anti-inflammatory cytokines. The local renal IR influenced distal organ apoptosis and inflammation by releasing circulating pro-inflammatory cytokines. The Non-IR and dorsal slit method for kidney surgery in a mouse model can be an alternative surgical approach for researchers without adverse reactions such as apoptosis, inflammation, fibrosis, functional impairment, and systemic reactions. English KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC CELL-DEATH APOPTOSIS EXPRESSION A Novel Dorsal Slit Approached Non-Ischemic Partial Nephrectomy Method for a Renal Tissue Regeneration in a Mouse Model Article 10.1007/s13770-018-0123-0 1 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.15, no.4, pp.453 - 466 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 15 4 453 466 scie scopus kci ART002371818 000440200400009 2-s2.0-85050534554 Cell & Tissue Engineering Engineering, Biomedical Cell Biology Engineering Article CELL-DEATH APOPTOSIS EXPRESSION Ischemia-reperfusion Renal regeneration Inflammation Apoptosis Fibrosis