Ryoo, Soo-Ryoon Yim, Yeajee Kim, Young-Kwan Park, Il-Soo Na, Hee-Kyung Lee, Jieon Jang, Hongje Won, Cheolhee Hong, Sungwoo Kim, Sung-Yon Jeon, Noo Li Song, Joon Myong Min, Dal-Hee 2024-01-19T22:04:30Z 2024-01-19T22:04:30Z 2021-09-03 2018-07-30 2045-2322 https://pubs.kist.re.kr/handle/201004/121129 MicroRNAs (miRNAs) are important regulatory RNAs that control gene expression in various biological processes. Therefore, control over the disease-related miRNA expression is important both for basic research and for a new class of therapeutic modality to treat serious diseases such as cancer. Here, we present a high-throughput screening strategy to identify small molecules that modulate miRNA expression in living cells. The screen enables simultaneous monitoring of the phenotypic cellular changes associated with the miRNA expression by measuring quantitative fluorescent signals corresponding to target miRNA level in living cells based on a novel biosensor composed of peptide nucleic acid and nano-sized graphene oxide. In this study, the biosensor based cellular screening of 967 compounds (including FDA-approved drugs, enzyme inhibitors, agonists, and antagonists) in cells identified four different classes of small molecules consisting of (i) 70 compounds that suppress both miRNA-21 (miR-21) expression and cell proliferation, (ii) 65 compounds that enhance miR-21 expression and reduce cell proliferation, (iii) 2 compounds that suppress miR-21 expression and increase cell proliferation, and (iv) 21 compounds that enhance both miR-21 expression and cell proliferation. We further investigated the hit compounds to correlate cell morphology changes and cell migration ability with decreased expression of miR-21. English NATURE PUBLISHING GROUP CANCER MIR-21 OVEREXPRESSION PROLIFERATION PROFILES TARGETS ACID RNA High-throughput chemical screening to discover new modulators of microRNA expression in living cells by using graphene-based biosensor Article 10.1038/s41598-018-29633-x 1 SCIENTIFIC REPORTS, v.8 SCIENTIFIC REPORTS 8 scie scopus 000440144400030 2-s2.0-85050798001 Multidisciplinary Sciences Science & Technology - Other Topics Article CANCER MIR-21 OVEREXPRESSION PROLIFERATION PROFILES TARGETS ACID RNA 그래핀 바이오센서 유전자