Shin, Jong-Il Jeon, Yong-Joon Lee, Sol Lee, Yoon Gyeong Kim, Ji Beom Kwon, Hak Cheol Kim, Sung Hun Kim, Inki Lee, Kyungho Han, Ye Sun 2024-01-19T22:30:39Z 2024-01-19T22:30:39Z 2022-01-25 2018-07 2314-6133 https://pubs.kist.re.kr/handle/201004/121175 Rhewnatoid arthritis (RA) is a chronic autoimmune inflammatory disease characterized by synovitis, hyperplasia, and the destruction of bone and cartilage. A variety of immunosuppressive biological agents have been developed because the pathogenesis of RA is related predominantly to the inflammatory response. However, rheumatoid arthritis fibroblast-like synovial cells (RAFLS), which are known to play an important role in RA progression, exhibit resistance to immunosuppressants through cancer-like properties. In this study, we identified a novel therapeutic compound for RA, which reduced inflammation and the abnormal proliferation of RAFLS in natural product library made from Korean native plants. Eupatorium japonicum Thunb. (EJT) extract, a component of the natural product library, most effectively reduced viability through the induction of ROS-mediated apoptosis in a dose-dependent manner. In addition, the increased ROS induced the expression of ATF4 and CHOP, key players in ER stress-mediated apoptosis. Interestingly, EJT extract treatment dose-dependently reduced the expression of IL-1 beta and the transcription of MM P-9, which were induced by TNF-alpha treatment, through the inhibition of NF-kappa B and p38 activation. Collectively, we found that EJT extract exerted apoptotic effects through increases in ROS production and CHOP expression and exerted anti-inflammatory effects through the suppression of NF-kappa B activation, IL-1 beta expression, and MMP-9 transcription. English HINDAWI LTD Apoptotic and Anti-Inflammatory Effects of Eupatorium japonicum Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes Article 10.1155/2018/1383697 1 BIOMED RESEARCH INTERNATIONAL, v.2018 BIOMED RESEARCH INTERNATIONAL 2018 Y scie scopus 000439224900001 2-s2.0-85050945049 Biotechnology & Applied Microbiology Medicine, Research & Experimental Biotechnology & Applied Microbiology Research & Experimental Medicine Article ENDOPLASMIC-RETICULUM STRESS ACTIVATED PROTEIN-KINASE NF-KAPPA-B ER-STRESS OXIDATIVE STRESS NATURAL-PRODUCTS TNF-ALPHA P38 MAPK IN-VITRO CELLS