Shafiq, Muhammad Zhang, Qiuying Zhi, Dengke Wang, Kai Kong, Deling Kim, Dong-Hwee Kim, Soo Hyun 2024-01-19T22:30:44Z 2024-01-19T22:30:44Z 2021-09-03 2018-07 1079-5642 https://pubs.kist.re.kr/handle/201004/121180 Objective The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1-derived peptide were fabricated by electrospinning. SP and SDF-1 peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1 and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1 grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1 grafts. Conclusions SDF-1 and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations. English LIPPINCOTT WILLIAMS & WILKINS MUSCLE PROGENITOR CELLS LARGE ANIMAL-MODEL TISSUE REGENERATION MACROPHAGE POLARIZATION MYOCARDIAL-INFARCTION SAPHENOUS-VEIN LOCAL-DELIVERY STEM-CELLS RECRUITMENT ENDOTHELIALIZATION In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1 Peptide Eluting Vascular Grafts Article 10.1161/ATVBAHA.118.310934 1 ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, v.38, no.7, pp.E117 - E134 ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 38 7 E117 E134 scie scopus 000436412900002 2-s2.0-85054811503 Hematology Peripheral Vascular Disease Hematology Cardiovascular System & Cardiology Article MUSCLE PROGENITOR CELLS LARGE ANIMAL-MODEL TISSUE REGENERATION MACROPHAGE POLARIZATION MYOCARDIAL-INFARCTION SAPHENOUS-VEIN LOCAL-DELIVERY STEM-CELLS RECRUITMENT ENDOTHELIALIZATION endothelial cells regeneration stem cells substance P tissue engineering