Kim, Kyoung-Ran Rothlisberger, Pascal Kang, Seong Jae Nam, Kihwan Lee, Sangyoup Hollenstein, Marcel Ahn, Dae-Ro 2024-01-19T22:30:47Z 2024-01-19T22:30:47Z 2021-09-03 2018-07 1420-3049 https://pubs.kist.re.kr/handle/201004/121183 Rolling circle amplification (RCA) is a robust way to generate DNA constructs, which are promising materials for biomedical applications including drug delivery because of their high biocompatibility. To be employed as a drug delivery platform, however, the DNA materials produced by RCA need to be shaped into nanoparticles that display both high cellular uptake efficiency and nuclease resistance. Here, we showed that the DNA nanoparticles (DNPs) can be prepared with RCA and modified nucleotides that have side-chains appended on the nucleobase are capable of interacting with the DNA strands of the resulting RCA products. The incorporation of the modified nucleotides improved cellular uptake efficiency and nuclease resistance of the DNPs. We also demonstrated that these DNPs could be employed as carriers for the delivery of a photosensitizer into cancer cells to achieve photodynamic therapy upon irradiation at both the in vitro and in vivo levels. English MDPI PHOTODYNAMIC THERAPY METHYLENE-BLUE NONPORPHYRIN PHOTOSENSITIZERS OLIGONUCLEOTIDES CYTOTOXICITY PORPHYRIN CANCER TUMORS SIZE Shaping Rolling Circle Amplification Products into DNA Nanoparticles by Incorporation of Modified Nucleotides and Their Application to In Vitro and In Vivo Delivery of a Photosensitizer Article 10.3390/molecules23071833 1 MOLECULES, v.23, no.7 MOLECULES 23 7 scie scopus 000445301800336 2-s2.0-85052022350 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article PHOTODYNAMIC THERAPY METHYLENE-BLUE NONPORPHYRIN PHOTOSENSITIZERS OLIGONUCLEOTIDES CYTOTOXICITY PORPHYRIN CANCER TUMORS SIZE rolling circle amplification DNA nanoparticles modified nucleotides photosensitizer photodynamic therapy