Binh Thanh Nguyen Park, Min Yoo, Young Sook Kang, Min-Jung 2024-01-19T22:31:07Z 2024-01-19T22:31:07Z 2021-09-03 2018-07 0003-2654 https://pubs.kist.re.kr/handle/201004/121202 We developed an assay for diagnosing rheumatoid arthritis (RA) by capillary electrophoresis with the laser-induced fluorescence detection (CE-LIF) of antibodies against cyclic citrullinated peptides (CCPs) using fluorescein isothiocyanate-labeled F-CCP11A and F-CCP. The CCPs were incubated overnight with a purified antibody against CCP and then injected into the CE-LIF system to estimate complex concentration. The immunocomplexes and free peptides were separated within 5 min, the LOD and LOQ values of the immunoassay are 0 1 mu g mL(-1) and 0 3 mu g mL(-1), respectively. The calibration curves for the immunocomplex had a coefficient of determination of 0.98. The reproducibility of the CE-LIF based immunoassay when using F-CCP11A and F-CCP was less than 5% and the accuracy ranged between 89 and 103%. The applicability of the assay was evaluated using serum samples from RA patients and healthy control subjects. We found that the sensitivity and specificity for RA diagnosis were 100% and 83.3%, respectively, for F-CCP11A and 100% and 90%, respectively, for F-CCP. These results demonstrate that the CE-LIF method can be useful for quantitative immunoassay development. English Royal Society of Chemistry Capillary electrophoresis-laser-induced fluorescence (CE-LIF)-based immunoassay for quantifying antibodies against cyclic citrullinated peptides Article 10.1039/c8an00714d 1 Analyst, v.143, no.13, pp.3141 - 3147 Analyst 143 13 3141 3147 N scie scopus 000441407700022 2-s2.0-85049072874 Chemistry, Analytical Chemistry Article EARLY RHEUMATOID-ARTHRITIS COMPETITIVE IMMUNOASSAY ANTIPERINUCLEAR FACTOR PROTEIN SERUM LIF AUTOANTIBODY STRATEGIES DISEASE MARKER anti-CCP CE-LIF Immunoassay RA diagnosis