Kim, H.Y. Jang, J.-E. Ahn, D.-R. 2024-01-19T22:31:38Z 2024-01-19T22:31:38Z 2021-09-02 2018-07 2576-6422 https://pubs.kist.re.kr/handle/201004/121230 Functional nucleic acids including siRNA, mRNA, and plasmid DNA are promising bioactive molecules to regulate cellular functions uncontrollable by conventional small molecule regulators. To realize successful cellular applications of these nucleic acids, an intracellular gene delivery vehicle with high efficiency and low cytotoxicity is required. Here, we report the dimerization of human β-defensin 3 (DhBD3) promoted by the interaction between β-strands and the application of DhBD3 for efficient delivery of various nucleic acid cargos. DhBD3 with multiple cationic residues could be complexed with various types of polyanionic DNA and RNA. DhBD3 could intracellularly deliver both small and large nucleic acid cargos loaded by complexation to regulate the expression level of target proteins, showing its potential as a universal platform for nucleic acid delivery. In addition, as DhBD3 is a human-derived material with high biocompatibility and can be robustly prepared by an inexpensive method, it is a promising gene delivery system that can be employed for biomedical purposes. ？ 2018 American Chemical Society. English American Chemical Society Biocompatibility Biomolecules Gene transfer Genes Cellular applications Defensins Functional nucleic acids Gene Delivery Intracellular delivery Intracellular gene delivery Nucleic acid deliveries Self aggregation Nucleic acids Dimeric Human β-Defensin 3 as a Universal Platform for Intracellular Delivery of Nucleic Acid Cargos Article 10.1021/acsabm.8b00024 1 ACS Applied Bio Materials, v.1, no.1, pp.100 - 109 ACS Applied Bio Materials 1 1 100 109 scopus 2-s2.0-85068335288 Article Biocompatibility Biomolecules Gene transfer Genes Cellular applications Defensins Functional nucleic acids Gene Delivery Intracellular delivery Intracellular gene delivery Nucleic acid deliveries Self aggregation Nucleic acids dimeric β-defensin 3 gene delivery vehicles nucleic acid cargos self-aggregation β-defensin