Cho, Eunji Nam, Gi-Hoon Hong, Yeonsun Kim, Yoon Kyoung Kim, Dong-Hwee Yang, Yoosoo Kim, In-San 2024-01-19T22:32:11Z 2024-01-19T22:32:11Z 2021-09-03 2018-06-10 0168-3659 https://pubs.kist.re.kr/handle/201004/121255 Exosomes are small membrane vesicles secreted by most cell types that play an important role in intercellular communication. Due to the characteristic of transferring their biomacromolecules, exosomes have potential as a new alternative for delivering protein therapeutics. Here, we investigate whether exosomes provide crucial advantages over other nanoparticles, in particular protein nanocage formulations, as a delivery system for membrane protein therapeutics. We characterized membrane-scaffold-based exosomes and protein-scaffold-based ferritin nanocages, both harboring SIRP alpha (signal regulatory protein alpha), an antagonist of CD47 on tumor cells. The efficacy of these two systems in delivering protein therapeutics was compared by testing their ability to enhance phagocytosis of tumor cells by bone-marrow-derived macrophages and subsequent inhibition of in vivo tumor growth. These analyses allowed us to comprehensively conclude that the therapeutic index of exosome-mediated CD47 blockade against tumor growth inhibition was higher than that of the same dose of ferritin-SIRP alpha. The results of this analysis reveal the importance of the unique characteristics of exosomes, in particular their membrane scaffold, in improving therapeutic protein delivery compared with protein-scaffold-based nanocages. English ELSEVIER SCIENCE BV CANCER-CELL PHAGOCYTOSIS DRUG-DELIVERY SIRP-ALPHA CD47 NANOPARTICLES PHARMACOKINETICS IMMUNOTHERAPY BLOCKADE PLATFORM BIODISTRIBUTION Comparison of exosomes and ferritin protein nanocages for the delivery of membrane protein therapeutics Article 10.1016/j.jconrel.2018.04.037 1 JOURNAL OF CONTROLLED RELEASE, v.279, pp.326 - 335 JOURNAL OF CONTROLLED RELEASE 279 326 335 scie scopus 000433211300030 2-s2.0-85046358597 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article CANCER-CELL PHAGOCYTOSIS DRUG-DELIVERY SIRP-ALPHA CD47 NANOPARTICLES PHARMACOKINETICS IMMUNOTHERAPY BLOCKADE PLATFORM BIODISTRIBUTION Exosome Membrane protein therapeutics Ferritin nanocages SIRP alpha CD47