Bastings, Maartje M. C. Anastassacos, Frances M. Ponnuswamy, Nandhini Leifer, Franziska G. Cuneo, Garry Lin, Chenxiang Ingber, Donald E. Ryu, Ju Hee Shih, William M. 2024-01-19T22:32:53Z 2024-01-19T22:32:53Z 2021-09-03 2018-06 1530-6984 https://pubs.kist.re.kr/handle/201004/121291 Designer nanoparticles with controlled shapes and sizes are increasingly popular vehicles for therapeutic delivery due to their enhanced cell-delivery performance. However, our ability to fashion nanoparticles has offered only limited control over these parameters. Structural DNA nanotechnology has an unparalleled ability to self-assemble three-dimensional nanostructures with near-atomic resolution features, and thus, it offers an attractive platform for the systematic exploration of the parameter space relevant to nanoparticle uptake by living cells. In this study, we examined the cell uptake of a panel of 11 distinct DNA-origami shapes, with the largest dimension ranging from 50-400 nm, in 3 different cell lines. We found that larger particles with a greater compactness were preferentially internalized compared with elongated, high-aspect-ratio particles. Uptake kinetics were also found to be more cell-type-dependent than shape-dependent, with specialized endocytosing dendritic cells failing to saturate over 12 h of study. The knowledge gained in the current study furthers our understanding of how particle shape affects cellular uptake and heralds the development of DNA nanotechnologies toward the improvement of current state-of-the-art cell-delivery vehicles. English AMER CHEMICAL SOC DRUG-DELIVERY PARTICLE DESIGN MAMMALIAN-CELLS NANOSTRUCTURES SIZE INTERNALIZATION NANOPARTICLES NANOMATERIALS MECHANISMS PATHWAYS Modulation of the Cellular Uptake of DNA Origami through Control over Mass and Shape Article 10.1021/acs.nanolett.8b00660 1 NANO LETTERS, v.18, no.6, pp.3557 - 3564 NANO LETTERS 18 6 3557 3564 scie scopus 000435524300035 2-s2.0-85047413125 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article DRUG-DELIVERY PARTICLE DESIGN MAMMALIAN-CELLS NANOSTRUCTURES SIZE INTERNALIZATION NANOPARTICLES NANOMATERIALS MECHANISMS PATHWAYS DNA origami nanotechnology cellular uptake nanoparticles structure-function relationship