Lee, GeonHui Lim, Jaeho Park, JiSoo Lee, Wonseok Yoon, Dae Sung Kim, Soo Hyun Kim, Myung-Ki Lee, Sang-Hoon Kim, Dong-Hwee 2024-01-19T22:33:13Z 2024-01-19T22:33:13Z 2021-09-03 2018-06 1226-086X https://pubs.kist.re.kr/handle/201004/121309 Developing a three-dimensional (3D) neural tissue model is important to comprehensively understand neural development and neuronal degeneration associated with various neurological disorders such as axonopathy and neuronopathy. Here, a new microplatform suitable for constructing neuronal spheroids (neurospheroids) was developed by modulating cell-surface interactions. The inner surface of a polydimethylsiloxane (PDMS) concave microwell array extensively used in in vitro cell aggregation was modified with typical extracellular matrix (ECM) molecules or carbon nanotubes to control neural spheroid formation. Modulating neuronal cell-ECM interactions could tune 3D intercellular interactions and spheroidal functionality. Neurite outgrowth, a neuronal marker for complex interneuronal signaling, was found to be tightly regulated by cell-ECM interactions in a confined space. Furthermore, amyloid-beta (A beta)-induced axonopathy representing a pathological feature of neurodegenerative diseases in vivo was examined in this study to monitor the degeneration of neurite outgrowth and alteration of neuronal morphology in these neurospheroids. The proposed neural tissue model could be used to study various neurodegenerative diseases in the future. (C) 2018 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. English 한국공업화학회 Construction of neurospheroids via surface modified concave microwells Article 10.1016/j.jiec.2018.01.014 1 Journal of Industrial and Engineering Chemistry, v.62, pp.341 - 351 Journal of Industrial and Engineering Chemistry 62 341 351 N scie scopus kci ART002356103 000431939700037 2-s2.0-85041561755 Chemistry, Multidisciplinary Engineering, Chemical Chemistry Engineering Article AMYLOID-BETA-PEPTIDE NEURAL STEM-CELLS CARBON NANOTUBES IN-VITRO APOLIPOPROTEIN-E CEREBRAL-CORTEX NERVOUS-SYSTEM GROWTH ADHESION NEURONS 3D neural tissues Cell-cell interactions Cell-extracellular matrix interactions Axonopathy