Akter, Hafeza Yoon, Jung Hwan Yoo, Young Sook Kang, Min-Jung 2024-01-19T22:33:45Z 2024-01-19T22:33:45Z 2021-09-03 2018-06 1016-8478 https://pubs.kist.re.kr/handle/201004/121338 Gastric cancer is the fifth most common type of malignancy worldwide, and the survival rate of patients with advanced-stage gastric cancer is low, even after receiving chemotherapy. Here, we validated neurotensin receptor 1 (NTSR1) as a potential therapeutic target in gastric cancer. We compared NTSR1 expression levels in sixty different gastric cancer-tissue samples and cells, as well as in other cancer cells (lung, breast, pancreatic, and colon), by assessing NTSR1 expression via semi-quantitative real-time reverse transcription polymerase chain reaction, immunocytochemistry and western blot. Following neurotensin (NT) treatment, we analyzed the expression and activity of matrix metalloproteinase-9 (MMP-9) and further determined the effects on cell migration and invasion via wound-healing and transwell assays. Our results revealed that NTSR1 mRNA levels were higher in gastric cancer tissues than non-cancerous tissues. Both of NTSR1 mRNA levels and expression were higher in gastric cancer cell lines relative to levels observed in other cancer-cell lines. Moreover, NT treatment induced MMP-9 expression and activity in all cancer cell lines, which was significantly decreased following treatment with the NTSR1 antagonist SR48692 or small-interfering RNA targeting NTSR1. Furthermore, NT-mediated metastases was confirmed by observing epithelial-mesenchymal transition markers SNAIL and E-cadherin in gastric cancer cells. NT-mediated invasion and migration of gastric cancer cells were reduced by NTSR1 depletion through the Erk signaling. These findings strongly suggested that NTR1 constitutes a potential therapeutic target for the inhibition of gastric cancer invasion and metastasis. English 한국분자세포생물학회 Validation of Neurotensin Receptor 1 as a Therapeutic Target for Gastric Cancer Article 10.14348/molcells.2018.0025 1 Molecules and Cells, v.41, no.6, pp.591 - 602 Molecules and Cells 41 6 591 602 N scie scopus kci ART002385183 000436198000011 2-s2.0-85056556580 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article ACTIVATED PROTEIN-KINASE GROWTH-FACTOR RECEPTOR CELL LUNG-CANCER BREAST-CANCER TUMOR PROGRESSION PROSTATE-CANCER RT-PCR EXPRESSION MMP-9 MATRIX-METALLOPROTEINASE-9 gastric cancer MMP-9 neurotensin NTSR1