Murale, Dhiraj P. Hong, Seong Cheol Haque, Md Mamunul Lee, Jun-Seok 2024-01-19T22:34:19Z 2024-01-19T22:34:19Z 2022-01-10 2018-05-18 1439-4227 https://pubs.kist.re.kr/handle/201004/121367 Glutathione (GSH) is one of major antioxidants inside cells that regulates oxidoreduction homeostasis. Recently, there have been extensive efforts to visualize GSH in live cells, but most of the probes available today are simple detection sensors and do not provide details of cellular localization. A new fluorescent probe (pcBD2-Cl), which is cell permeable and selectively reacts with GSH in situ, has been developed. The in situ GSH-labeled probe (pcBD2-GSH) exhibited quenches fluorescence, but subsequent binding to cellular abundant glutathione S-transferase (GST) recovers the fluorescence intensity, which makes it possible to image the GSH-GST complex in live cells. Interactions between probe and GST were confirmed by means of photo-crosslinking under intact live-cell conditions. Interestingly, isomers of chloro-functionalized 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) compounds behaved very distinctively inside the cells. Following co-staining imaging with MitoTracker and mitochondria fractionation upon lipopolysaccharide-mediated reactive oxygen species induction experiments showed that pcBD2-GSH accumulated in mitochondria. This is the first example of a live-cell imaging probe to visualize translocation of GSH from the cytosol to mitochondria. English WILEY-V C H VERLAG GMBH HIGHLY SELECTIVE DETECTION FLUORESCENT-PROBE S-TRANSFERASES MITOCHONDRIAL GLUTATHIONE 2-OXOGLUTARATE CARRIERS IN-VIVO THIOLS DICARBOXYLATE HOMOCYSTEINE CYSTEINE Chloro-Functionalized Photo-crosslinking BODIPY for Glutathione Sensing and Subcellular Trafficking Article 10.1002/cbic.201800059 1 CHEMBIOCHEM, v.19, no.10, pp.1001 - 1005 CHEMBIOCHEM 19 10 1001 1005 scie scopus 000432921600001 2-s2.0-85045845789 Biochemistry & Molecular Biology Chemistry, Medicinal Biochemistry & Molecular Biology Pharmacology & Pharmacy Article HIGHLY SELECTIVE DETECTION FLUORESCENT-PROBE S-TRANSFERASES MITOCHONDRIAL GLUTATHIONE 2-OXOGLUTARATE CARRIERS IN-VIVO THIOLS DICARBOXYLATE HOMOCYSTEINE CYSTEINE dyes pigments fluorescence imaging agents photochemistry sensors