Choi, Man Ho 2024-01-19T22:34:38Z 2024-01-19T22:34:38Z 2021-09-03 2018-05-05 0303-7207 https://pubs.kist.re.kr/handle/201004/121383 Owing to controversy over the effects of steroids on breast cancer pathophysiology, comprehensive quantification of steroid hormones has been extensively considered in both clinical practice and biomarker discovery studies. In contrast to the traditional immunoaffinity-based assays, which show cross-reactivity and have poor validity at low levels of sex steroids, mass spectrometry is becoming a promising tool for measuring steroid levels in complex biological specimens. The Endocrine Society has announced and continuously updated on technical advances to apply high-quality breakthroughs in the clinical sciences. To avoid incorrect estimation of the steroids of interest, however, further emphasis should be made on the efficient separation by chromatography, such as gas and liquid chromatography, prior to mass spectrometric (MS) detection. Recent advances in MS-based analysis of sex steroids associated with breast cancer enable accurate quantification of circulating as well as localized steroids from frozen tissue slices, allowing these assays to be more powerful in clinical practice. (C) 2017 Elsevier B.V. All rights reserved. English ELSEVIER IRELAND LTD POSTMENOPAUSAL WOMEN CHROMATOGRAPHY/MASS SPECTROMETRY ESTROGEN-LEVELS LC-MS/MS PREMENOPAUSAL WOMEN REFERENCE INTERVALS SERUM ESTROGENS HUMAN PLASMA ESTRADIOL HORMONES Mass spectrometry-based metabolic signatures of sex steroids in breast cancer Article 10.1016/j.mce.2017.09.023 1 MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.466, pp.81 - 85 MOLECULAR AND CELLULAR ENDOCRINOLOGY 466 81 85 scie scopus 000447948900008 2-s2.0-85029551338 Cell Biology Endocrinology & Metabolism Cell Biology Endocrinology & Metabolism Review POSTMENOPAUSAL WOMEN CHROMATOGRAPHY/MASS SPECTROMETRY ESTROGEN-LEVELS LC-MS/MS PREMENOPAUSAL WOMEN REFERENCE INTERVALS SERUM ESTROGENS HUMAN PLASMA ESTRADIOL HORMONES Sex steroid Mass spectrometry Chromatography Metabolite profiling Breast cancer