Chun, So Young Kim, Hyun Tae Ha, Yun-Sok Yu, Na-Hee Lih, Eugene Kim, Dae Hwan Kwon, Se Yun Song, Phil Hyun Kim, Jeongshik Kim, Bum Soo Kim, BupWan Han, Dong Keun Kwon, Tae Gyun 2024-01-19T22:34:44Z 2024-01-19T22:34:44Z 2022-01-25 2018-05 2157-9083 https://pubs.kist.re.kr/handle/201004/121389 Immunomodulation should be an important consideration when using polylactic acid-polyglycolic acid copolymer (PLGA) based scaffolds, because the initial acute immune response prevents tissue regeneration. To achieve initial immunomodulation, magnesium hydroxide [Mg(OH)(2)], which is known to neutralize the acidity resulting from the PLGA degradation products, was incorporated into the PLGA scaffold. To investigate the in vivo effect Mg(OH)(2), PLGA with Mg(OH)(2) (PLGA/MH) scaffolds were implanted into partial nephrectomy sites in mice, and morphological, histological, molecular-biological and immunohistochemical analyses were performed. The expression of T-cell, pro-inflammatory, and fibrotic factors was lower in the PLGA/MH group than the PLGA group. The PLGA/MH group exhibited a significant increase in host stem cell recruitment. The recruited cells differentiated in situ into renal cells and formed glomeruli. The recruited mesenchymal cells in PLGA/MH group exhibited high levels of anti-inflammatory cytokine expression compared to those in the PLGA group. These results support the concept that targeted tissue regeneration can be stimulated by initial control of the inflammatory and fibrotic microenvironment, and thereby enhancing the processes of host stem/progenitor cell recruitment, in situ differentiation, and anti-inflammatory cytokine secretion. English AMER SCIENTIFIC PUBLISHERS Initial Immunomodulation Using Mg(OH)(2) Promotes Renal Regeneration via Anti-Inflammatory Processes Article 10.1166/jbt.2018.1794 1 JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, v.8, no.5, pp.704 - 715 JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING 8 5 704 715 N scie 000438964900014 Cell & Tissue Engineering Cell Biology Article MESENCHYMAL STEM-CELLS INJURY TRANSPLANTATION PH Immunomodulation Anti-Inflammatory Host Cell Recruitment In Situ Differentiation Cytokine Target Tissue Regeneration Scaffold