Kim, J. S. Jang, H. J. Jeong, E. K. Kim, S. S. Kim, Y. M. Lee, J. H. Oh, M. Y. Lee, D. K. Yelithao, K. Kwan, H. C. Nho, C. W. Eom, D. W. 2024-01-19T23:00:35Z 2024-01-19T23:00:35Z 2021-09-03 2018-05 0041-1345 https://pubs.kist.re.kr/handle/201004/121417 Background. Crepidiastrum denticulatum (CD) is a well-known, traditionally consumed vegetable in Korea, which was recently reported to contain bioactive compounds with detoxification and antioxidant properties. Ischemia-reperfusion injury (IRI) is a major problem after renal transplantation. Furthermore, inflammatory responses to IRI exacerbate the resultant renal injury. In the present study, we investigated whether CD extract exhibits renoprotective effects against IR-induced acute kidney injury in mice. Materials and methods. Renal IRI was induced in male C57BL/6 mice by bilateral renal pedicle occlusion for 30 minutes followed by reperfusion for 48 hours. CD extract (75 mg/kg) was administered orally 5 days before IRI. Results. Treatment with CD extract significantly decreased blood urea nitrogen and serum creatinine levels as well as kidney tubular injury. CD also prevented IRI-induced renal glutathione depletion and increased malondialdehyde levels. Western blotting and reverse transcriptase polymerase chain reaction indicated that CD extract significantly attenuates inducible nitric oxide synthase and toll-like receptor 2/4 protein levels 48 h after IRI. The expression of tumor necrosis factor-alpha and interleukin-1 beta was significantly decreased in the CD extract treatment group. Conclusion. CD extract improved acute renal IRI through its antioxidant and anti-inflammatory effects. These findings suggest that CD extract is a potential therapeutic agent for acute ischemia-induced renal damage. English ELSEVIER SCIENCE INC ACUTE-RENAL-FAILURE TOLL-LIKE RECEPTOR-2 ISCHEMIA/REPERFUSION INJURY OXIDATIVE STRESS NATURAL-PRODUCTS CELLS PROTECTS INFLAMMATION DAMAGE INHIBITION Crepidiastrum denticulatum Extract Ameliorates Kidney Ischemia-Reperfusion Injury in Mice Article 10.1016/j.transproceed.2018.02.048 1 TRANSPLANTATION PROCEEDINGS, v.50, no.4, pp.1160 - 1166 TRANSPLANTATION PROCEEDINGS 50 4 1160 1166 scie scopus 000431940700040 2-s2.0-85046680991 Immunology Surgery Transplantation Immunology Surgery Transplantation Article; Proceedings Paper ACUTE-RENAL-FAILURE TOLL-LIKE RECEPTOR-2 ISCHEMIA/REPERFUSION INJURY OXIDATIVE STRESS NATURAL-PRODUCTS CELLS PROTECTS INFLAMMATION DAMAGE INHIBITION