Kim, Hyungsup Kim, Hyesu Lee, Jesun Lee, Byeongjun Kim, Hee-Ryang Jung, Jooyoung Lee, Mi-Ock Oh, Uhtaek 2024-01-19T23:00:41Z 2024-01-19T23:00:41Z 2021-09-03 2018-05 0143-4160 https://pubs.kist.re.kr/handle/201004/121423 Anoctamins (ANOs) are multifunctional membrane proteins that consist of 10 homologs. ANO1 (TMEM16A) and ANO2 (TMEM16B) are anion channels activated by intracellular calcium that meditate numerous physiological functions. ANO6 is a scramblase that redistributes phospholipids across the cell membrane. The other homologs are not well characterized. We found ANO9/TMEM16J is a cation channel activated by a cAMP-dependent protein kinase A (PKA). Intracellular cAMP-activated robust currents in whole cells expressing ANO9, which were inhibited by a PKA blocker. A cholera toxin that persistently stimulated adenylate cyclase activated ANO9 as did the application of PKA. The cAMP-induced ANO9 currents were permeable to cations. The cAMP-dependent ANO9 currents were augmented by intracellular Ca-2+. Ano9 transcripts were predominant in the intestines. Human intestinal SW480 cells expressed high levels of Ano9 transcripts and showed PKA inhibitor-reversible cAMP-dependent currents. We conclude that ANO9 is a cation channel activated by a cAMP/PKA pathway and could play a role in intestine function. English ELSEVIER SCI LTD CA2+-ACTIVATED CL-CHANNEL CHLORIDE CHANNEL THERAPEUTIC TARGET INTERSTITIAL-CELLS 1 TMEM16A EXPRESSION TUMORIGENESIS MUTATIONS SECRETION CURRENTS Anoctamin 9/TMEM16J is a cation channel activated by cAMP/PKA signal Article 10.1016/j.ceca.2017.12.003 1 CELL CALCIUM, v.71, pp.75 - 85 CELL CALCIUM 71 75 85 scie scopus 000430652400008 2-s2.0-85044371669 Cell Biology Cell Biology Article CA2+-ACTIVATED CL-CHANNEL CHLORIDE CHANNEL THERAPEUTIC TARGET INTERSTITIAL-CELLS 1 TMEM16A EXPRESSION TUMORIGENESIS MUTATIONS SECRETION CURRENTS Anoctamins PKA cAMP Cation channel Calcium