Ko, Ja Kyong Jung, Je Hyeong Altpeter, Fredy Kannan, Baskaran Kim, Ha Eun Kim, Kyoung Heon Alper, Hal S. Um, Youngsoon Lee, Sun-Mi 2024-01-19T23:00:57Z 2024-01-19T23:00:57Z 2021-09-03 2018-05 0960-8524 https://pubs.kist.re.kr/handle/201004/121437 The recalcitrant structure of lignocellulosic biomass is a major barrier in efficient biomass-to-ethanol bioconversion processes. The combination of feedstock engineering via modification in the lignin synthesis pathway of sugarcane and co-fermentation of xylose and glucose with a recombinant xylose utilizing yeast strain produced 148% more ethanol compared to that of the wild type biomass and control strain. The lignin reduced biomass led to a substantially increased release of fermentable sugars (glucose and xylose). The engineered yeast strain efficiently co-utilized glucose and xylose for fermentation, elevating ethanol yields. In this study, it was experimentally demonstrated that the combined efforts of engineering both feedstock and microorganisms largely enhances the bioconversion of lignocellulosic feedstock to bioethanol. This strategy will significantly improve the economic feasibility of lignocellulosic biofuels production. English Elsevier BV Largely enhanced bioethanol production through the combined use of lignin-modified sugarcane and xylose fermenting yeast strain Article 10.1016/j.biortech.2018.01.123 1 Bioresource Technology, v.256, pp.312 - 320 Bioresource Technology 256 312 320 N scie scopus 000428043000040 2-s2.0-85042182732 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article IMPROVES ETHANOL-PRODUCTION SACCHAROMYCES-CEREVISIAE BIOFUEL PRODUCTION SIMULTANEOUS SACCHARIFICATION DIRECTED EVOLUTION ACETIC-ACID FERMENTATION PRETREATMENT LIGNOCELLULOSE RECALCITRANCE Lignocellulosic bioethanol Biomass recalcitrance Lignin modification Xylose utilizing strain Co-fermentation