Kim, Tae Hee Jung, Youngmee Kim, Soo Hyun 2024-01-19T23:01:00Z 2024-01-19T23:01:00Z 2021-09-03 2018-05 1937-3341 https://pubs.kist.re.kr/handle/201004/121440 Produced through electrospinning, poly(l-lactide-co-caprolactone) (PLCL) membranes, which have a porous structure and are biodegradable, are of interest in various medical fields. The porous-structured electrospun membrane is particularly interesting because of several favorable properties as follows: it exudes fluid from the wound, does not build up under the wound covering, and does not cause wound desiccation. Moreover, extracellular matrix (ECM)-based structures derived by tissue decellularization have application as engineered tissue scaffolds and as supports for cellular regeneration. In particular, heart decellularized ECM (hdECM) has various pro-angiogenic factors that can induce angiogenesis for wound healing. In this regard, a nanofibrous electrospun hdECM-based hybrid scaffold (NEhdHS), which is a PLCL membrane, including hdECM as an active agent, was tested as a wound dressing to assess its fundamental biochemical and physical features in wound healing. Use of NEhdHS with its porous structure and pro-angiogenic factors is expected to provide an effective wound dressing and reduced scarring. We first demonstrate the effectiveness of a proposed decellularization protocol through analysis of dECM components and describe the mechanical properties of the fabricated NEhdHS. Next, we present an in vitro angiogenesis analysis of the NEhdHS, using a coculture system with human dermal fibroblasts and human umbilical vein endothelial cells; the results of which confirm its biocompatibility and show that the NEhdHS can significantly enhance angiogenesis over that obtained from PLCL or gelatin-containing PLCL scaffolds. We also studied the effectiveness of the NEhdHS in vivo. Using a rat excisional wound-splinting model, we show that covering the upper part of the wound with NEhdHS significantly reduces scarring in the wound healing process compared to that with PLCL or gelatin-containing PLCL scaffolds. Based upon its properties, we conclude that the NEhdHS has potential for application in wound dressing. English MARY ANN LIEBERT, INC MACROPHAGE PHENOTYPES BACTERIAL CELLULOSE IN-VITRO TISSUE CHITOSAN ANGIOGENESIS MECHANISMS GROWTH SKIN NANOPARTICLES Nanofibrous Electrospun Heart Decellularized Extracellular Matrix-Based Hybrid Scaffold as Wound Dressing for Reducing Scarring in Wound Healing Article 10.1089/ten.tea.2017.0318 1 TISSUE ENGINEERING PART A, v.24, no.9-10, pp.830 - 848 TISSUE ENGINEERING PART A 24 9-10 830 848 scie scopus 000419555000001 2-s2.0-85047205968 Cell & Tissue Engineering Cell Biology Engineering, Biomedical Materials Science, Biomaterials Cell Biology Engineering Materials Science Article MACROPHAGE PHENOTYPES BACTERIAL CELLULOSE IN-VITRO TISSUE CHITOSAN ANGIOGENESIS MECHANISMS GROWTH SKIN NANOPARTICLES heart decellularized extracellular matrix angiogenesis wound healing scarless wound dressing