Seo, Jungmok Shin, Jung-Youn Leijten, Jeroen Jeon, Oju Ozturk, Ayca Bal Rouwkema, Jeroen Li, Yuancheng Shin, Su Ryon Hajiali, Hadi Alsberg, Eben Khademhosseini, Ali 2024-01-19T23:01:23Z 2024-01-19T23:01:23Z 2021-09-03 2018-04-25 1944-8244 https://pubs.kist.re.kr/handle/201004/121458 Biophysical cues can potently direct a cell's or tissue's behavior. Cells interpret their biophysical surroundings, such as matrix stiffness or dynamic mechanical stimulation, through mechanotransduction. However, our understanding of the various aspects of mechanotransduction has been limited by the lack of proper analysis platforms capable of screening three-dimensional (3D) cellular behaviors in response to biophysical cues. Here, we developed a dynamic compression bioreactor to study the combinational effects of biomaterial composition and dynamic mechanical compression on cellular behavior in 3D hydrogels. The bioreactor contained multiple actuating posts that could apply cyclic compressive strains ranging from 0 to 42% to arrays of cell-encapsulated hydrogels. The bioreactor could be interconnected with other compressive bioreactors, which enabled the combinatorial screenings of 3D cellular behaviors simultaneously. As an application of the screening platform, cell spreading, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) were characterized in 3D gelatin methacryloyl (GelMA) hydrogels. Increasing hydrogel concentration from S to 10% restricted the cell spreading, however, dynamic compressive strain increased cell spreading. Osteogenic differentiation of hMSCs was also affected by dynamic compressive strains. hMSCs in 5% GelMA hydrogel were more sensitive to strains, and the 42% strain group showed a significant increase in osteogenic differentiation compared to other groups. The interconnectable dynamic compression bioreactor provides an efficient way to study the interactions of cells and their physical microenvironments in three dimensions. English American Chemical Society EMBRYONIC STEM-CELLS BONE-MARROW MECHANICAL STIMULATION OSTEOGENIC DIFFERENTIATION BIOMATERIAL MICROARRAYS STRAIN BEHAVIOR DESIGN GROWTH MICROENVIRONMENTS Interconnectable Dynamic Compression Bioreactors for Combinatorial Screening of Cell Mechanobiology in Three Dimensions Article 10.1021/acsami.7b17991 1 ACS Applied Materials & Interfaces, v.10, no.16, pp.13293 - 13303 ACS Applied Materials & Interfaces 10 16 13293 13303 scie scopus 000431150900006 2-s2.0-85045883824 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article EMBRYONIC STEM-CELLS BONE-MARROW MECHANICAL STIMULATION OSTEOGENIC DIFFERENTIATION BIOMATERIAL MICROARRAYS STRAIN BEHAVIOR DESIGN GROWTH MICROENVIRONMENTS high-throughput screening human mesenchymal stem cells dynamic compression bioreactor 3D mechanobiology mechanical stimulation